An efficient immobilized denitrification bioreactor functioning under anaerobic conditions was developed by combining bacterial immobilization technology with iron-carbon (Fe–C) particles. The effects of key factors on nitrate (NO 3 − –N) removal efficiency were invested, such as the carbon-nitrogen ratio (C/N), pH and hydraulic retention time (HRT). Experimental results show that 100.00% NO 3 − –N removal efficiency and a low level of nitrite (NO 2 − –N) accumulation less than 0.05 mg L −1 were obtained under the condition of a C/N ratio of 3, pH 7.0 and HRT of 6 h. Meteorological chromatographic analysis showed that the final product of denitrification was mainly nitrogen (N 2 ). The main component of precipitation formed in the bioreactor was characterized as Fe 3 O 4 by X-ray diffraction. High-throughput sequencing analysis indicated that the dominant bacterial class in the Fe–C bioreactor was Gammaproteobacteria , while the dominant genera were Zoogloea and Azospira , the relative abundances of which were as high as 23.25 and 15.43%, respectively.

中文翻译：

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新型PVA /铁碳（Fe–C）固定化生物反应器用于去除地下水中硝酸盐的性能和微生物群落

通过将细菌固定化技术与铁-碳（Fe-C）颗粒相结合，开发了一种在厌氧条件下起作用的高效固定式反硝化生物反应器。研究了关键因素对硝酸盐（NO 3--N）去除效率的影响，例如碳氮比（C / N），pH和水力停留时间（HRT）。实验结果表明，在C / N比为3，pH 7.0的条件下，获得了100.00％的NO 3--N去除效率和低于0.05 mg L -1的少量亚硝酸盐（NO 2--N）积累。和HRT为6小时。气象色谱分析表明，反硝化的最终产物主要是氮（N 2）。通过X射线衍射将在生物反应器中形成的沉淀的主要成分表征为Fe 3 O 4。