The present study investigated the acute effects of three key environmental factors (temperature, pH, and suspended solids) on the physiology of the boring giant clam Tridacna crocea. Single‐factor experiments for a duration time of 10 h showed that high thermal stress (25 [control], 28, and 30°C), water acidification (pH 8.0 [control], 7.5, and 7.0), and suspended solids (no sands [control], or coral sand with grain size either 0.1 or 0.5 mm) all increased the mRNA expressions of heat‐shock‐protein genes and apoptosis‐related genes, respectively, in the gills of giant clams. Both water acidification and suspended solids also up‐regulated lactic acid dehydrogenase and glutamic‐pyruvate transaminase enzyme activities. There was no significant variation among the different treatment conditions in the digestive enzyme (trypsin, lipase, and amylase) activities in the hepatopancreas of giant clams, except that significant higher lipase activities were observed in those that were exposed to 28°C and pH 7.0. The present results imply that temperature, pH, and suspended solids are important environmental conditions that affect the physiology of giant clams during large‐scale artificial breeding.

中文翻译：

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温度，pH和悬浮固体对无聊大C Tridacna crocea抗氧化和消化酶活性及应激反应基因表达的急性影响

本研究调查了三个关键环境因素（温度，pH和悬浮固体）对无聊大蛤Tri大戟（Tridacna crocea）生理的急性影响。。单因素实验持续10小时，显示出高热应力（25 [对照]，28和30°C），水酸化（pH 8.0 [对照]，7.5和7.0）和悬浮固体（无）。沙粒（对照）或粒径为0.1或0.5 mm的珊瑚沙）分别增加了巨蛤c中热休克蛋白基因和凋亡相关基因的mRNA表达。水酸化和悬浮固体都上调了乳酸脱氢酶和谷氨酸丙酮酸转氨酶的活性。在不同处理条件下，大蛤lam的肝胰脏的消化酶（胰蛋白酶，脂肪酶和淀粉酶）活性之间没有显着差异，不同的是，暴露于28°C和pH 7.0的那些中脂肪酶活性显着提高。 。