The plant growth promoting rhizobacterium (PGPR) Bacillus amyloliquefaciens (strain GB03) augments Arabidopsis glucosinolate defenses targeting the generalist herbivore Spodoptera exigua. With the goal to transfer such technology to an agricultural crop, bacterial-defense induction was monitored in the closely related species, Eruca sativa (arugula). Plant growth promotion and leaf protection against the herbivore specialist Plutella xylostella (diamondback-moth) was induced with GB03-seed inoculation. PGPR-induced herbivore protection correlated with greater gene induction encoding for glucosinolate biosynthesis. To monitor rhizosphere-specific GB03 proliferation, PGPR-specific primers were designed for qPCR gene amplification. Rhizosphere colonization was detected with GB03 inoculation for 28 days, while rhizosphere GB03 colonization diminished to below detection level after 21 days for insect-damaged plants. By selectively monitoring GB03 from a complex mix of soil microbes with seed inoculation, rhizosphere PGPR proliferation and foliar glucosinolate transcription for plant protection against the diamondback-moth specialist can be monitored in vivo under authentic field conditions.

促进植物生长的根瘤菌（PGPR）解淀粉芽孢杆菌（菌株GB03）增强了针对通才草食性斜纹夜蛾的拟南芥芥子油苷的防御能力。为了将这种技术转移到农作物上，在密切相关的物种Eruca sativa（芝麻菜）中监测了细菌防御的诱导作用。对抗草食动物小菜蛾的植物生长促进和叶片保护用GB03种子接种诱导（菱蛾）。PGPR诱导的草食动物保护与芥子油苷生物合成的更大的基因诱导编码有关。为了监测根际特异性GB03的增殖，设计了PGPR特异性引物用于qPCR基因扩增。用GB03接种28天后可检测到根际定植，而对虫害植株而言，在21天后，根际GB03的定植量降至检测水平以下。通过从土壤微生物与种子接种的复杂混合物中选择性监测GB03，可以在真实田间条件下在体内监测根际PGPR增殖和芥子油苷叶面转录，以保护植物免受小菜蛾专家的侵害。