Doxorubicin (DOX) is a potent anti-cancer antibiotic that was widely used for treatment of various cancers. It produces free radicals which result in extreme dose-limiting cardiotoxicity. This study investigated the cardioprotective potential of chia seed oil, an active polyphenolic nutraceutical against doxorubicin-induced cardiotoxicity in Wistar rats. Twenty-four female Wistar rats were divided into four groups (n = 6) which consist of normal control, DOX control, test-A and test-B group. Animals were prophylactically treated with two different doses of test drug, i.e. chia seed oil 2.5 ml/kg/day and 5 ml/kg/day in test-A and test-B groups orally for 7 days. Doxorubicin (25 mg/kg; single dose) was administered intraperitoneally to DOX control, Test-A and Test-B animals on the seventh day to induce cardiotoxicity. ECG analysis was done before and after treatment. Besides ECG, CK, CK-MB, LDH, AST, MDA and GSH were analyzed. DOX had significantly altered ECG, CK, CK-MB, LDH, AST, MDA and GSH. Pre-treatment with chia seed oil significantly alleviated DOX-induced ECG changes and also guarded against DOX-induced rise of serum CK, CK-MB and AST levels. Chia seed oil alleviated histopathological alteration in DOX-treated rats. It also significantly inhibited DOX-induced GSH depletion and elevation of MDA. The present study revealed that chia seed oil exerts cardioprotection against doxorubicin-induced cardiotoxicity in female Wistar rats. Our study opens the perspective to clinical studies to precisely consider chia seed oil as a potential chemoprotectant nutraceutical in the combination chemotherapy with doxorubicin to limit its cardiotoxicity.

阿霉素（DOX）是一种有效的抗癌抗生素，广泛用于治疗各种癌症。它产生自由基，导致极端的剂量限制性心脏毒性。本研究调查了奇亚籽油的心脏保护潜力，奇亚籽油是一种活性多酚营养保健品，可对抗阿霉素诱导的 Wistar 大鼠心脏毒性。 24只雌性Wistar大鼠分为4组（ n =6），即正常对照组、DOX对照组、试验A组和试验B组。在测试A组和测试B组中，用两种不同剂量的测试药物（即奇亚籽油2.5ml/kg/天和5ml/kg/天）对动物进行预防性治疗，持续7天。在第七天对 DOX 对照、测试 A 和测试 B 动物腹膜内施用阿霉素（25 mg/kg；单剂量）以诱导心脏毒性。在治疗前和治疗后进行心电图分析。除心电图外，还分析了 CK、CK-MB、LDH、AST、MDA 和 GSH。 DOX 显着改变心电图、CK、CK-MB、LDH、AST、MDA 和 GSH。用奇亚籽油进行预处理可显着减轻 DOX 引起的心电图变化，并防止 DOX 引起的血清 CK、CK-MB 和 AST 水平升高。奇亚籽油减轻了 DOX 治疗大鼠的组织病理学改变。它还显着抑制 DOX 诱导的 GSH 消耗和 MDA 升高。本研究表明，奇亚籽油对雌性 Wistar 大鼠具有心脏保护作用，抵抗阿霉素诱导的心脏毒性。我们的研究为临床研究开辟了新的视角，以精确地将奇亚籽油视为与阿霉素联合化疗中的潜在化学保护营养保健品，以限制其心脏毒性。