当前位置: X-MOL 学术Biocontrol › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Tracking the movement of Nesidiocoris tenuis among banker plants and crops in a tomato greenhouse by DNA markers of host plants
BioControl ( IF 2.2 ) Pub Date : 2021-03-19 , DOI: 10.1007/s10526-021-10085-8
Ryohei Nakano , Norihide Hinomoto

The zoophytophagous predator, Nesidiocoris tenuis (Reuter) (Hemiptera: Miridae), is an important biological control agent. To maintain this insect, several non-crop host plants are used as banker plants in greenhouse crop systems. To optimize the efficiency of the predator-banker plant interaction, it is necessary to investigate how individual predators move between banker plants and crops. However, the movement is difficult to quantify under field conditions. Therefore, we investigated the movement of N. tenuis between tomato plants (Solanum lycopersicum L., Solanales: Solanaceae) and three banker plants (Cleome hassleriana Chod., Brassicales: Cleomaceae; Sesamum indicum L., Lamiales: Pedaliaceae; and Verbena × hybrida Voss, Lamiales: Verbenaceae) in a greenhouse by conducting PCR using plant-species-specific primers. Laboratory analysis results showed that our molecular method could detect N. tenuis activity within a relatively short time (≤ 24 h). In addition, N. tenuis predation on a pest species was unlikely to result in false detection of plant DNA in the pest (suggesting that N. tenuis had been on the plants). Multiple plant species were detected in adult insects collected from the greenhouse plants, indicating that N. tenuis frequently moved across the mentioned plant species. The movement patterns of N. tenuis between plant species varied substantially based on the plant species from which they were collected, which suggested each of the plant species had different functions for N. tenuis. Our findings revealed that planting multiple host plants would stabilize the N. tenuis population in biological control programs.



中文翻译:

通过宿主植物的DNA标记追踪奈瑟球菌在番茄温室中银行植物和农作物之间的运动

食虫性捕食者,Nesidiocoris tenuis(Reuter)(Hemiptera:Miridae),是一种重要的生物防治剂。为了维持这种昆虫,温室作物系统中使用了几种非农作物寄主植物作为库房植物。为了优化捕食者与河岸植物互动的效率,有必要研究个体捕食者如何在河岸植物与农作物之间移动。但是,在野外条件下很难对运动进行量化。因此,我们研究的移动N.细叶番茄植物(间番茄L.,茄目:茄科)和三个银行家植物(白花菜hassleriana施身法,白花菜:醉蝶花科;芝麻L.,唇形目:胡麻科;以及温室中的马鞭草×杂交种(马鞭草科(Verbenaceae)),通过使用植物种特异性引物进行PCR进行分析。实验室分析结果表明,我们的分子方法可以在相对较短的时间内(≤24小时)检测到猪笼草的活性。此外,对虫害物种的特异猪笼草捕食不太可能导致在虫害中错误检测到植物DNA(暗示特异猪笼草已存在于植物上)。在从温室植物中收集的成虫中检测到多种植物物种,这表明特异奈瑟氏球菌经常在上述植物物种中移动。Tenuis猪笼草的运动模式植物物种之间的差异因收集植物物种的不同而有很大差异,这表明每种植物物种对特有猪笼草都有不同的功能。我们的研究结果表明,种植多种寄主植物将在生物防治计划中稳定特异猪笼草的种群。

更新日期:2021-03-19
down
wechat
bug