Bipolaris sorokiniana is a fungal pathogen that caused root rot and spot blotch on wheat. A Recombinase Polymerase Amplification (RPA) assay was developed to detect B. sorokiniana specifically based on the sequences of calmodulin gene sequence. The specificity of RPA assay was tested against 19 fungal organisms associated with wheat, and the detection limit was determined to be 10 pg for B. sorokiniana pure fungal DNA. Further, the RPA assay was capable of detecting B. sorokiniana from artificially inoculated and field collected wheat tissues directly. These results indicate that the RPA assay is rapid and effective method for detection and molecular identification of B. sorokiniana.

猪双极双歧杆菌是一种真菌病原体，可导致小麦的根腐烂和斑点斑点。开发了重组酶聚合酶扩增（RPA）测定法，以特别根据钙调蛋白基因序列的序列来检测索氏芽孢杆菌。测试了针对19种与小麦相关的真菌生物的RPA分析的特异性，对索氏假单胞菌纯真菌DNA的检出限确定为10 pg 。此外，RPA测定法能够直接从人工接种的和田间采集的小麦组织中检测出B. sorokiniana。这些结果表明，RPA法是一种快速，有效的检测和分子鉴定B. sorokiniana的方法。