ABSTRACT

Tissue factor (TF) has been extensively studied for tumor metastasis, but its role in mediating cancer cell adhesion to vasculature remains unknown. This study aimed to measure the ability of TF to mediate the adhesion of breast cancer cells to human umbilical vein endothelial cells (HUVECs). MDA-MB-231 cells expressed the highest TF level and adhered more to HUVECs under static and flow conditions, a neutralizing TF antibody abolished the enhanced adhesion of MDA-MB-231 cells to HUVECs. Recombinant human soluble TF (rTF) bonded β1integrin on HUVECs surfaces, β1 or α3integrin antibody combined with TF antibody abolished more cell-cell adhesion. These data suggested that TF mediated adhesion of breast cancer cells to endothelial cells may rely on β1integrin on HUVECs surfaces.

摘要

组织因子 (TF) 已被广泛研究用于肿瘤转移，但其在介导癌细胞粘附到脉管系统中的作用仍然未知。本研究旨在测量 TF 介导乳腺癌细胞与人脐静脉内皮细胞 (HUVEC) 粘附的能力。MDA-MB-231 细胞表达最高的 TF 水平，并且在静态和流动条件下更多地粘附到 HUVEC，中和 TF 抗体消除了 MDA-MB-231 细胞对 HUVEC 的增强粘附。重组人可溶性 TF (rTF) 结合 HUVECs 表面的 β1integrin，β1 或 α3integrin 抗体与 TF 抗体结合消除了更多的细胞 - 细胞粘附。这些数据表明 TF 介导的乳腺癌细胞与内皮细胞的粘附可能依赖于 HUVEC 表面的 β1 整合素。