Abstract

Objective

This study was designed to investigate whether the glucose lowering effects of Potentilla fulgens acts by modulating GLUT4, AKT2 and AMPK expression in the skeletal muscle and liver tissues.

Methodology

Alloxan-induced diabetic mice treated with Potentilla fulgens was assessed for their blood glucose and insulin level, mRNA and protein expression using distinguished methods. Additionally, GLUT4, AKT2 and AMPK were docked with catechin, epicatechin, kaempferol, metformin, quercetin and ursolic acid reportedly present in Potentilla fulgens.

Results

Potentilla fulgens ameliorates hyperglycaemia and insulin sensitivity via activation of AKT2 and AMPK, increases the expression of GLUT4, AKT2, AMPKα1 and AMPKα2 whose levels are reduced under diabetic condition. Molecular docking revealed interacting residues and their binding affinities (−4.56 to −8.95 Kcal/mol).

Conclusions

These findings provide more clarity vis-avis the mechanism of action of the phytoceuticals present in Potentilla fulgens extract which function through their action on GLUT4, PKB and AMPK.

中文翻译：

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委陵菜上调四氧嘧啶诱导的糖尿病小鼠中的 GLUT4、AMPK、AKT 和胰岛素：一项体内和计算机研究

摘要

客观的

本研究旨在探讨委陵菜的降血糖作用是否通过调节骨骼肌和肝组织中的 GLUT4、AKT2 和 AMPK 表达来发挥作用。

方法

使用显着方法评估四氧嘧啶诱导的糖尿病小鼠的血糖和胰岛素水平、mRNA 和蛋白质表达。此外，GLUT4、AKT2 和 AMPK 与据报道存在于委陵菜中的儿茶素、表儿茶素、山奈酚、二甲双胍、槲皮素和熊果酸对接。

结果

委陵菜通过激活 AKT2 和 AMPK 改善高血糖和胰岛素敏感性，增加 GLUT4、AKT2、AMPKα1 和 AMPKα2 的表达，而糖尿病条件下这些蛋白的水平会降低。分子对接揭示了相互作用的残基及其结合亲和力（-4.56 至 -8.95 Kcal/mol）。

结论

这些发现使委陵菜提取​​物中植物药物的作用机制更加清晰，这些植物药物通过作用于 GLUT4、PKB 和 AMPK 发挥作用。