Podosphaera xanthii is the main causal agent of powdery mildew in cucurbits and, arguably, the most important fungal pathogen of cucurbit crops. Here, we present the first reference genome assembly for P. xanthii. We performed a hybrid genome assembly, using reads from Illumina NextSeq550 and PacBio Sequel S3. The short and long reads were assembled into 1,727 scaffolds with an N₅₀ size of 163,173 bp, resulting in a 142-Mb genome size. The combination of homology-based and ab initio predictions allowed the prediction of 14,911 complete genes. Repetitive sequences comprised 76.2% of the genome. Our P. xanthii genome assembly improves considerably the molecular resources for research on P. xanthii−cucurbit interactions and provides new opportunities for further genomics, transcriptomics, and evolutionary studies in powdery mildew fungi.

Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Podosphaera xanthii是葫芦科白粉病的主要病原体，并且可以说是葫芦科作物最重要的真菌病原体。在这里，我们提出了黄单胞菌的第一个参考基因组组装。我们使用Illumina NextSeq550和PacBio Sequel S3的读段进行了杂交基因组装配。短读和长读被组装成1,727个N ₅₀大小为163,173 bp的支架，从而产生了142 Mb的基因组大小。基于同源性和从头算的预测相结合，可以预测14,911个完整基因。重复序列占基因组的76.2％。我们的黄腐病菌基因组装配极大地改善了黄腐病菌研究的分子资源葫芦相互作用，为白粉病真菌的进一步基因组学，转录组学和进化研究提供了新的机会。

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