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Morpho-physiological investigations in transgenic tomato ( Solanum lycopersicum L.) over expressing OsRGLP1 gene
In Vitro Cellular & Developmental Biology - Plant ( IF 2.2 ) Pub Date : 2021-03-17 , DOI: 10.1007/s11627-021-10165-5
Sumera Perveen , Tehmina Ahsan Khan , Humaira Shaheen , Rabia Naz , Muhammad Zeeshan Hyder , Bushra Ijaz , S. M. Saqlan Naqvi , Tayyaba Yasmin

The OsRGLP1 gene was overexpressed under the control of CaMV 35S promoter in tomato (Solanum lycopersicum L.) plants using Agrobacterium-mediated transformation. The transgene expression was validated using RT-qPCR (real-time quantitative polymerase chain reaction) in the transgenic plants. Noticeable morpho-physiological traits, higher SOD activity, and elevated histological H2O2 were manifested by transgenic tomato lines. An increase in total chlorophyll and relative water content (RWC) in the leaves of transgenic lines was noted. The electrolytic leakage/conductivity (EC) in leaves of transgenic lines was lower than the control. The transgenic tomato lines showed dwarf phenotypic features in vegetative and floral traits. Interestingly, the vascular cambium regions in cross sections of stem showed secondary growth in radial symmetry. The transgenic lines also produced higher density and diversity of trichomes. The differences observed in transgenic lines are quite interesting which need to be further explored referring to the functional aspects of the transgene with reference to stress.



中文翻译:

表达OsRGLP1基因的转基因番茄的形态生理研究

所述OsRGLP1基因在番茄CaMV 35S启动子的控制下过表达(番茄使用L.)植物的农杆菌介导的转化。使用RT-qPCR(实时定量聚合酶链反应)在转基因植物中验证了转基因表达。明显的形态生理特征,较高的SOD活性和较高的组织学H 2 O 2通过转基因番茄品系表现出来。注意到转基因品系叶片中总叶绿素和相对水分含量(RWC)的增加。转基因品系叶片中的电解渗漏/电导率(EC)低于对照。转基因番茄品系在营养和花卉性状上表现出矮小的表型特征。有趣的是,茎横断面上的血管形成层区域在径向对称性上表现出二次生长。转基因品系还产生了较高的毛状体密度和多样性。在转基因品系中观察到的差异是非常有趣的,需要参照转基因的功能方面和压力进行进一步探讨。

更新日期:2021-03-18
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