SummaryThe objective of this study was to investigate the effect of oocyte and sperm cryopreservation on donated eggs submitted to intracytoplasmic sperm injection (ICSI) cycles. Medical charts of 122 oocyte recipients undergoing 152 oocyte recipient ICSI cycles, from 2017 to 2018, in a private university-affiliated in vitro fertilization (IVF) centre, were reviewed in this historical cohort study. Cycles were divided into four groups according to the gamete status: the FO/FS Group, recipients in which fresh oocytes were injected with fresh sperm (n = 19); the FO/CrS Group, recipients in which fresh oocytes were injected with cryopreserved sperm (n = 14); the CrO/FS Group, recipients in which cryopreserved oocytes were injected with fresh sperm (n = 85); and the CrO/CrS Group, recipients in which cryopreserved oocytes were injected with cryopreserved sperm (n = 34). Generalized mixed models fit by restricted maximum likelihood, followed by Bonferroni post hoc test for the comparison of means amongst groups were used to investigate the effect of cryopreservation on recipient ICSI outcomes. The results were expressed as mean differences with 95% confidence intervals and P-values. The main outcome measure was the implantation rate. Normal day 3 cleavage speed, blastocyst development and implantation rates were significantly lower in the CrO/CrS Group compared with the FO/FS Group. In conclusion, embryo developmental competence and implantation potential were reduced when vitrified oocytes were injected with frozen sperm in an egg-sharing donation programme.

中文翻译：

---

雄性和雌性配子的冷冻保存导致胚胎发育和植入潜力降低

摘要本研究的目的是调查卵母细胞和精子冷冻保存对接受胞浆内精子注射 (ICSI) 周期的捐赠卵子的影响。从 2017 年到 2018 年，在一所私立大学附属机构中接受 152 个卵母细胞接受者 ICSI 周期的 122 名卵母细胞接受者的医疗图表体外受精（IVF）中心，在这项历史队列研究中进行了回顾。根据配子状态将周期分为四组：FO / FS组，新鲜卵母细胞注射新鲜精子的受者（n= 19); FO/CrS 组，在新鲜卵母细胞中注射冷冻保存的精子的受者（n= 14); CrO/FS 组，冷冻卵母细胞被注射新鲜精子的受者（n= 85); 和 CrO/CrS 组，冷冻保存的卵母细胞被注射冷冻保存的精子的受者（n= 34)。通过限制最大似然拟合的广义混合模型，然后是用于比较组间平均值的 Bonferroni 事后检验，用于研究冷冻保存对接受者 ICSI 结果的影响。结果表示为具有 95% 置信区间的平均差和磷-价值观。主要结果测量是植入率。与 FO/FS 组相比，CrO/CrS 组的正常第 3 天卵裂速度、囊胚发育和着床率显着降低。总之，当在卵子共享捐赠计划中向玻璃化卵母细胞注射冷冻精子时，胚胎发育能力和植入潜力会降低。