Big bud caused by several different phytoplasmas is an emerging threat to tomato production worldwide. The development of resistant varieties would be an effective approach to manage this problem, but it requires an appropriate screening technique. Recently, we have described a simple and efficient chip graft inoculation assay (CGIA) for the first time to screen tomato germplasm against Tomato leaf curl New Delhi virus. The present study was conducted to first validate the CGIA for phytoplasma transmission, then to assess the resistance of 74 genotypes belonging to different Solanum species against 16SrII-D phytoplasma. CGIA success rate and phytoplasma transmission was 100% since all the grafts survived and phytoplasma was detected in these plants using nested polymerase chain reaction. No genotype was found resistant as all the grafted plants showed typical disease symptoms. In addition to phytoplasma transmission, CGIA can be used for better understanding the plant–phytoplasma interactions and biology of phytoplasmas in tomato.

中文翻译：

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验证芯片嫁接接种试验以评估茄属植物对植原体的抗性

由几种不同植原体引起的大芽是对全球番茄生产的新威胁。开发抗性品种将是解决这一问题的有效方法，但它需要适当的筛选技术。最近，我们首次描述了一种简单有效的芯片移植接种试验（CGIA），用于筛选番茄种质对抗番茄卷叶新德里病毒。本研究首先验证了植原体传播的 CGIA，然后评估了 74 种不同基因型的抗性。茄属种抗 16SrII-D 植原体。CGIA 成功率和植原体传播率为 100%，因为所有的移植物都存活下来，并且使用嵌套聚合酶链式反应在这些植物中检测到了植原体。由于所有嫁接的植物都表现出典型的疾病症状，因此没有发现基因型具有抗性。除植原体传播外，CGIA 还可用于更好地了解番茄中植物与植原体的相互作用和植原体生物学。