Background Owing to neurite promoting, antioxidant and anti-inflammatory effects of Euxanthone (Eux), the investigation was aimed to probe the neuroprotective efficacy of Eux against traumatic spinal cord injury (t-SCI) in rats and whether Eux can improve neuropathic function in t-SCI. Method Sprague-Dawley (SD) rats were randomized in – Sham, t-SCI, Eux30, and Eux60 (t-SCI + 30 and 60 mg/kg respectively). Animals with compression force-induced t-SCI were subjected to estimation of locomotor functions. Spinal cord water content and Evans blue (EB) effusion were determined for quantifying edema and intactness of the spinal cord. Oxidative stress and immunochemical markers were quantified by ELISA and western blotting. Results Findings revealed that Eux60 group animals had greater Basso, Beattie, and Bresnahan (BBB) and (incline plane test) IPT score indicating improved locomotor functions. There was a reduction in the spinal edema and water content after Eux treatment, together with lowering of oxidative stress markers. The expression of IL-6, IL-12, IL-1β, caspase-3, RANKL, TLR4, NF-κB, p-38, PI3K, and Akt in spinal cord tissues of t-SCI-induced rats was lowered after Eux treatment. Conclusion Overall, the investigation advocates that Eux attenuates t-SCI and associated inflammation, oxidative damage, and resulting apoptosis via modulation of TLR4/NF-κB/p38 and PI3K/Akt signaling cascade.

中文翻译：

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Euxanthone 通过抗氧化应激和抑制大鼠模型中的 p38 和 PI3K/Akt 信号通路抑制创伤性脊髓损伤


背景由于Euxanthone（Eux）具有促进神经突、抗氧化和抗炎作用，本研究旨在探讨Eux对大鼠创伤性脊髓损伤（t-SCI）的神经保护作用以及Eux是否可以改善大鼠的神经病理功能。 -SCI。方法 Sprague-Dawley (SD) 大鼠被随机分为 Sham、t-SCI、Eux30 和 Eux60（t-SCI 分别 + 30 和 60 mg/kg）。对患有压缩力诱导的 t-SCI 的动物进行运动功能评估。测定脊髓含水量和伊文思蓝（EB）积液以量化脊髓的水肿和完整性。通过 ELISA 和蛋白质印迹法对氧化应激和免疫化学标记物进行定量。结果结果显示，Eux60 组动物具有更高的 Basso、Beattie 和 Bresnahan (BBB) 和（斜面测试）IPT 评分，表明运动功能得到改善。 Eux 治疗后脊髓水肿和水含量有所减少，同时氧化应激标志物也有所降低。 Eux后t-SCI诱导大鼠脊髓组织中IL-6、IL-12、IL-1β、caspase-3、RANKL、TLR4、NF-κB、p-38、PI3K和Akt的表达降低治疗。结论 总体而言，该研究主张 Eux 通过调节 TLR4/NF-κB/p38 和 PI3K/Akt 信号级联来减轻 t-SCI 和相关炎症、氧化损伤以及由此产生的细胞凋亡。