Abstract

Here, we studied the protective effect of gallic acid (GAL) as a potent anti-oxidant and anti-inflammatory agent against damage caused by busulfan (BUS) in the testes of adult rats. The adult Wistar rats were assigned as control, BUS: was intraperitoneally (i.p.) treated with busulfan (15 mg/kg, day 7 and 14), GAL + BUS: was co-treated with busulfan (i.p., 15 mg/kg, day 7 and 14) and orally treated (per os) with gallic acid (60 days, 20 mg/kg) and GAL: was treated with gallic acid (per os, 60 days, 20 mg/kg). The results showed that GAL co-treatment increased the numbers of spermatogonia (Type A and B), spermatocytes (primary and secondary) and round spermatids, along with the tubular diameter, epithelial height and gonado-somatic index. In addition, BUS-induced increase in 3β-hydroxysteroid dehydrogenase and γ-glutamyl transpeptidase activities were inhibited on GAL co-treatment. Similarly, BUS-induced decrease in gluthathione concentration, catalase and superoxide dismutase activities along with increase in myeloperoxidase activity and malondialdehyde concentration were significantly normalized to control values on GAL co-treatment. Busulfan-induced elimination of tubular germ cells was completely prevented by GAL. Overall, GAL may inhibit BUS-mediated spermatogenesis arrest via decreasing inflammatory-mediated oxidative stress in a rat experimental model.

摘要

在这里，我们研究了没食子酸 (GAL) 作为一种有效的抗氧化剂和抗炎剂对成年大鼠睾丸中白消安 (BUS) 造成的损伤的保护作用。成年 Wistar 大鼠被指定为对照，BUS：用白消安（15 mg/kg，第 7 天和第 14 天）腹膜内（ip）治疗 ，GAL + BUS：用白消安联合治疗（ip， 15  mg/kg，天7 和 14) 并用没食子酸 (60天, 20 mg/kg) 和 GAL口服治疗 ( per os) : 用没食子酸治疗 ( per os , 60天, 20    毫克/公斤）。结果表明，GAL 联合治疗增加了精原细胞（A 型和 B 型）、精母细胞（初级和次级）和圆形精子细胞的数量，以及管径、上皮高度和性腺体细胞指数。此外，在 GAL 联合治疗中，BUS 诱导的 3β-羟基类固醇脱氢酶和 γ-谷氨酰转肽酶活性的增加受到抑制。类似地，BUS 诱导的谷胱甘肽浓度、过氧化氢酶和超氧化物歧化酶活性的降低以及髓过氧化物酶活性和丙二醛浓度的增加显着标准化为 GAL 联合治疗的对照值。GAL 完全阻止了白消安诱导的管状生殖细胞消除。全面的，