Coronavirus causes an infectious disease in various species and crosses the species barriers leading to the outbreak of zoonotic diseases. Due to the respiratory diseases are mainly caused in humans and viruses are replicated and excreted through the respiratory tract, the nasal fluid and sputum are mainly used for diagnosis. Early diagnosis of coronavirus plays an important role in preventing its spread and is essential for quarantine policies. For rapid decision and prompt triage of infected host, the immunochromatographic assay (ICA) has been widely used for point of care testing. However, when the ICA is applied to an expectorated sputum in which antigens are present, the viscosity of sputum interferes with the migration of the antigens on the test strip. To overcome this limitation, it is necessary to use a mucolytic agent without affecting the antigens. In this study, we combined known mucolytic agents to lower the viscosity of sputum and applied that to alpha and beta coronavirus, porcine epidemic diarrhea virus (PEDV) and Middle East respiratory syndrome coronavirus (MERS-CoV), respectively, spiked in sputum to find optimal pretreatment conditions. The pretreatment method using tris(2-carboxyethyl)phosphine (TCEP) and BSA was suitable for ICA diagnosis of sputum samples spiked with PEDV and MERS-CoV. This sensitive assay for the detection of coronavirus in sputum provides an useful information for the diagnosis of pathogen in low respiratory tract.

中文翻译：

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侧流免疫层析法检测冠状病毒的痰处理方法。

冠状病毒可引起多种物种的传染病，并越过物种壁垒导致人畜共患疾病的爆发。由于呼吸道疾病主要是人类引起的，病毒是通过呼吸道复制和排泄的，因此鼻液和痰液主要用于诊断。冠状病毒的早期诊断在防止其传播中起着重要作用，对于隔离政策至关重要。为了快速确定感染宿主并快速分流，免疫色谱分析（ICA）已广泛用于即时检验。然而，当将ICA应用于存在抗原的痰液中时，痰液的粘度会干扰抗原在试纸上的迁移。为了克服这个限制，必须使用不影响抗原的粘液溶解剂。在这项研究中，我们结合了已知的粘液溶解剂来降低痰液的黏度，并将其分别应用到掺入痰液中的α和β冠状病毒，猪流行性腹泻病毒（PEDV）和中东呼吸综合征冠状病毒（MERS-CoV）中最佳预处理条件。使用三（2-羧乙基）膦（TCEP）和BSA的预处理方法适用于ICA诊断PEDV和MERS-CoV加标的痰标本。这种检测痰中冠状病毒的灵敏方法为诊断下呼吸道病原体提供了有用的信息。猪流行性腹泻病毒（PEDV）和中东呼吸综合征冠状病毒（MERS-CoV）分别掺入痰液中以找到最佳的治疗条件。使用三（2-羧乙基）膦（TCEP）和BSA的预处理方法适用于ICA诊断PEDV和MERS-CoV加标的痰标本。这种检测痰中冠状病毒的灵敏方法为诊断下呼吸道病原体提供了有用的信息。猪流行性腹泻病毒（PEDV）和中东呼吸综合征冠状病毒（MERS-CoV）分别掺入痰液中以找到最佳的治疗条件。使用三（2-羧乙基）膦（TCEP）和BSA的预处理方法适用于ICA诊断PEDV和MERS-CoV加标的痰标本。这种检测痰中冠状病毒的灵敏方法为诊断下呼吸道病原体提供了有用的信息。