Kinetics of hepatitis A virus (HAV) accumulation and depuration from mussels (Mytilus galloprovincialis) was studied in an experimental depuration system. Different parameters likely to influence the rate of virus accumulation and elimination were evaluated. Analyses were carried out by both real-time RT-qPCR and digital PCR. Results demonstrated that the animals start to concentrate the virus already after one hour and reach the maximum level of contamination in 6 h of experiment. With respect to depuration, HAV showed a rapid reduction of the concentration (89%) during the first 24–48 h of experiment and a very slow virus decrement in the following days with a 1% residual RNA at the ninth day of depuration. When process parameters likely to increase the depuration rate (presence of ozone, microalgal feeding, presence of lactic bacteria, pre-treatment with digestive enzymes) were tested, no significant differences in the kinetics were observed. Only treatment with pancreatin seemed to positively affect depuration in the first two days of the experiment.

贻贝甲型肝炎病毒 (HAV) 积累和净化的动力学 ( Mytilus galloprovincialis )) 在实验净化系统中进行了研究。评估了可能影响病毒积累和消除速率的不同参数。通过实时 RT-qPCR 和数字 PCR 进行分析。结果表明，动物在一小时后已经开始浓缩病毒，并在实验的 6 小时内达到最大污染水平。在净化方面，HAV 显示在实验的前 24-48 小时内浓度迅速降低（89%），随后几天病毒减少非常缓慢，在净化的第 9 天残留 RNA 为 1%。当测试可能提高净化率的工艺参数（臭氧的存在、微藻进料、乳酸菌的存在、用消化酶预处理）时，没有观察到动力学的显着差异。