Fruit Brix is an important indicator in determining the quality of tomatoes (Solanum lycopersicum), and increasing it is an important objective. The production of high Brix tomatoes requires breeding and genetic studies of fruit. During domestication S. lycopersicum lost genetic variation of some wild tomato relative that could be useful for breeding. In this study, we investigated introgression lines (ILs) from a cross between the wild relative Solanum pennellii and the cultivated tomato S. lycopersicum ‘M82’. While there are many genetic and physiological studies that demonstrate the usefulness of tomato S. pennellii ILs, few have investigated the high Brix values of IL fruit. Accordingly, we attempted to detect tomato ILs that resulted in high Brix ripening fruit, in order to obtain valuable genetic and genomic resources for the investigation of phenotypes originating in the S. pennellii genome. IL5-4 may be a line that carries an S. pennellii chromosome segment on chromosome 5 of ‘M82’. Previous research indicated that IL5-4 fruit have higher Brix levels than ‘M82’ fruit. Our results corroborated these findings and revealed Brix changes in fruit during development. We also found that IL5-4 plants showed a higher incidence of blossom-end rot (BER), a major physiological disorder in tomatoes. Therefore, we investigated the physiological mechanism responsible for the higher incidence of BER in IL5-4, by focusing on calcium content, which may be related to BER occurrence. The total and water-soluble Ca contents of fruit tissues were significantly lower in IL5-4 than in ‘M82’ in the proximal part, while no differences were observed in the distal part. Thus, our results suggested that a higher incidence of BER in IL5-4 fruit may not be related to both total and water-soluble Ca contents in the distal fruit tissue, and genetic factors originating in the S. pennellii chromosome may induce high BER incidence in IL5-4. The characterization of IL5-4 in this study showed that it is a valuable genetic and genomic resource for high-Brix breeding stock and for the investigation of novel BER mechanisms.

水果糖度是确定番茄质量的重要指标，而增加番茄糖含量是一个重要目标。高糖度番茄的生产需要水果的育种和遗传研究。在驯化过程中，番茄裂殖酵母失去了一些野生番茄亲缘种的遗传变异，可能对育种有用。在这项研究中，我们调查了野生近缘茄和栽培番茄S. lycopersicum'M82 '之间杂交的渗入系（ILs）。尽管有许多遗传和生理学研究证明了番茄S.pennellii的有用性IL，很少有人研究过IL水果的高糖度值。因此，我们试图检测导致高糖度成熟水果的番茄白介素，以获取有价值的遗传和基因组资源，以调查源自彭氏葡萄球菌基因组的表型。IL5-4可以是携带一个线S.潘那利番茄“ M82”的5号染色体上的染色体片段。先前的研究表明，IL5-4水果的白利糖度水平高于“ M82”水果。我们的结果证实了这些发现，并揭示了果实在发育过程中糖度的变化。我们还发现，IL5-4植物显示出较高的开花期腐烂（BER）发生率，后者是番茄的主要生理疾病。因此，我们通过关注钙含量（可能与BER的发生有关）来研究导致IL5-4中BER发生率较高的生理机制。在IL5-4中，果实组织的总钙和水溶性Ca含量在近端部分显着低于“ M82”，而在远端部分没有观察到差异。因此，潘那利番茄S.染色体可能诱发高BER发生率IL5-4。IL5-4在这项研究中的表征表明，它是高白利糖度育种和研究新型BER机制的宝贵遗传和基因组资源。