This study utilized the principle that the bacteriorhodopsin (BR) produced by Halobacterium salinarum could increase the hydrogen production of Rhodobacter sphaeroides. H. salinarum are co-cultured with R. sphaeroides to determine the impact of purple membrane fragments (PM) on R. sphaeroides and improve its hydrogen production capacity. In this study, low-salinity in 14 % NaCl domesticates H salinarum. Then, 0–160 nmol of different concentration gradient groups of bacteriorhodopsin (BR) and R. sphaeroides was co-cultivated, and the hydrogen production and pH are measured; then, R. sphaeroides and immobilized BR of different concentrations are used to produce hydrogen to detect the amount of hydrogen. Two-chamber microbial hydrogen production system with proton exchange membrane-assisted proton flow was established, and the system was operated. As additional electricity added under 0.3 V, the hydrogen production rate increased with voltages in the coupled system. H salinarum can still grow well after low salt in 14% NaCl domestication. When the BR concentration is 80 nmol, the highest hydrogen production reached 217 mL per hour. Both immobilized PC (packed cells) and immobilized PM (purple membrane) of H. salinarum could promote hydrogen production of R. sphaeroides to some extent. The highest production of hydrogen was obtained by the coupled system with 40 nmol BR of immobilized PC, which increased from 127 to 232 mL, and the maximum H2 production rate was 18.2 mL−1 h−1 L culture. In the 192 h experiment time, when the potential is 0.3 V, the hydrogen production amount can reach 920 mL, which is 50.3% higher than the control group. The stability of the system greatly improved after PC was immobilized, and the time for hydrogen production of R. sphaeroides significantly extended on same condition. As additional electricity added under 0.3 V, the hydrogen production rate increased with voltages in the coupled system. These results are helpful to build a hydrogen production-coupled system by nitrogenase of R. sphaeroides and proton pump of H. salinarum.

中文翻译：

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增强制氢的类球红细菌细胞外ħ促进⁺的嗜盐杆菌

本研究利用食盐盐杆菌产生的细菌视紫红质（BR）可以增加球形红细菌的产氢量的原理。将盐假单胞菌与球形红球菌共培养以确定紫色膜碎片（PM）对球形红球菌的影响并提高其产氢能力。在这项研究中，低盐度的14％NaCl驯化了盐沼。然后，将细菌视紫红质（BR）和球形红球菌的不同浓度梯度组的0-160 nmol共同培养，并测量产氢量和pH值；然后，使用不同浓度的球形红球菌和固定化的BR生产氢气，以检测氢气量。建立了具有质子交换膜辅助质子流的两室微生物制氢系统，并对该系统进行了操作。当在0.3 V以下添加额外的电时，氢气生成速率随耦合系统中的电压而增加。低盐含量的14％NaCl驯化后，盐沼H仍然可以很好地生长。当BR浓度为80 nmol时，最高产氢量达到每小时217 mL。盐沼嗜血菌的固定化PC（堆积细胞）和固定化PM（紫色膜）均可在一定程度上促进球形红球菌的产氢。通过与40 nmol BR固定化PC偶联的系统获得的氢气最高产量，从127 mL增加到232 mL，最大H2产生速率为18.2 mL-1 h-1 L培养物。在192 h实验时间内，当电势为0.3 V时，制氢量可达到920 mL，比对照组高50.3％。固定PC后，系统的稳定性大大提高，并且在相同条件下，球形红球菌产氢的时间显着延长。当在0.3 V以下添加额外的电时，制氢速度随着耦合系统中的电压而增加。这些结果有助于通过球形红球菌的固氮酶和盐沼的质子泵建立制氢耦合系统。