ABSTRACT

Overworking and overloading of donkeys may cause fatigue. The experiment aims to determine the effects of epicatechin-(4β-8)-catechin (EEC) on some fatigue biomarkers in packed donkeys during the dry season. 10 jacks, aged between four and five years were used for the study. Five were given EEC in feed seven days before packing (test), while another five were given feed only (control). Blood samples collected for biochemical analyses were analyzed for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), total antioxidant capacity (TAC), creatine kinase (CK) and tumor necrosis factor-alpha (TNF-α). The SOD activity increased significantly (P < 0.05) in the test group during the cold-dry season after packing. The values of TAC during the packing period were significantly (P < 0.05) higher in test group during the hot-dry season. The TNF-α concentration was higher (P > 0.05) in the control group especially one-hour on route. The CK activity was significantly (P < 0.05) higher in the control donkeys during the hot-dry season. In conclusion, packing donkeys during the dry season resulted in fatigue, but EEC served as an anti-fatigue agent.

摘要

过度劳累和超负荷的驴可能会导致疲劳。该实验旨在确定表儿茶素-(4β-8)-儿茶素 (EEC) 对旱季驴群疲劳生物标志物的影响。研究使用了 10 个年龄在 4 到 5 岁之间的千斤顶。5 只在包装前 7 天在饲料中添加 EEC（测试），而另外 5 只只饲喂（对照）。收集用于生化分析的血样分析超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、谷胱甘肽过氧化物酶 (GPx)、总抗氧化能力 (TAC)、肌酸激酶 (CK) 和肿瘤坏死因子-α (TNF-α)。包装后寒冷干燥季节试验组SOD活性显着升高（P < 0.05）。包装期间TAC值显着（P < 0. 05) 在干热季节测试组较高。对照组的 TNF-α 浓度较高（P > 0.05），尤其是在途中一小时。在炎热干燥季节，对照驴的CK活性显着升高（P <0.05）。总之，在旱季打包驴会导致疲劳，但 EEC 起到了抗疲劳剂的作用。