A simple, rapid, sensitive and reproducible method was developed for the simultaneous quantification of four bioactive compounds, quercetin, gallic acid, eugenol and β-sitosterol, by high-performance thin-layer chromatography (HPTLC) in Psidium guajava L. HPTLC was performed on silica gel 60 F₂₅₄ by using toluene‒ethyl acetate‒formic acid (7:3:1, V/V) for quercetin and gallic acid and toluene‒ethyl actetate‒formic acid (9:1:0.5, V/V) for β-sitosterol and eugenol as the mobile phase, at retention factor values of 0.52 ± 0.06, 0.28 ± 0.05, 0.87 ± 0.02 and 0.59 ± 0.04 for quercetin, gallic acid, eugenol and β-sitosterol, respectively. The quantifications of gallic acid, quercetin, eugenol and β-sitosterol were carried out using a densitometric absorption mode at 254 nm, 370 nm, 570 nm and 600 nm. The developed method was validated and found to be specific, linear and accurate with precision and accuracy in the concentration range of 200‒1000 ng/mL for gallic acid, eugenol and β-sitosterol and 100‒500 ng/mL for quercetin. Precision studies (both inter-day and intra-day) are within the standard limit of RSD (%) less than 3% (Table 1). The contents of the marker compounds, viz., quercetin (0.0031), gallic acid (0.223), eugenol (0.0052%) and β-sitosterol (0.0466) % dry weight basis were detected in of Psidium guajava. The statistical data analysis showed that the method is reproducible and selective for the estimation of all the four bioactive compounds in extracts of Psidium guajava.

建立了一种简单，快速，灵敏且可重现的方法，用于在番石榴中通过高效薄层色谱（HPTLC）同时定量四种槲皮素，没食子酸，丁子香酚和β-谷甾醇的生物活性化合物。用甲苯‒乙酸乙酯‒甲酸（7：3：1，V / V）在硅胶60 F ₂₅₄上制备槲皮素和没食子酸，以及甲苯act乙酸乙酯（甲酸（9：1：0.5，V / V）以β-谷固醇和丁子香酚为流动相，槲皮素，没食子酸，丁子香酚和β-谷甾醇的保留因子分别为0.52±0.06、0.28±0.05、0.87±0.02和0.59±0.04。没食子酸，槲皮素，丁子香酚和β-谷固醇的定量使用光密度吸收模式在254 nm，370 nm，570 nm和600 nm进行。经验证，所开发的方法特异，线性且准确，没食子酸，丁子香酚和β-谷甾醇的浓度范围为200‒1000 ng / mL，槲皮素的浓度范围为100‒500 ng / mL。精密度研究（日间和日内）均在RSD（％）的标准范围内，小于3％（表1）。标记化合物的含量，即在番石榴中检测到了槲皮素（0.0031），没食子酸（0.223），丁子香酚（0.0052％）和β-谷甾醇（0.0466）％干重。统计数据分析表明，该方法对番石榴提取物中的四种生物活性化合物的估计均具有重现性和选择性。