A screening of commercially available lipases for the synthesis of vitamin E succinate showed that lipase from Candida rugosa presented the highest yield. The synthesis of vitamin E succinate in organic solvents with different lgP values ranging from −1.3 to 3.5 was investigated. Of particular interest was that dimethyl sulfoxide (DMSO) with the lowest lgP exhibited the highest yield among all the organic solvents used. It suggests that lgP is incapable of satisfactorily predicting the biocompatibility of organic solvents due to the complexity of enzymatic reaction with hydrophilic and hydrophobic substrates in organic solvent. Effects of different operating conditions, such as molar ratio of substrate, enzyme concentration, reaction temperature, mass transfer, and reaction time were also studied. Under the optimum conditions of 10 g/L enzyme, a stirring rate of 100 r/min, a substrate molar ratio of 5:1 at 55 °C for 18 h, a satisfactory yield(46.95%) was obtained. The developed method has a potential to be used for efficient enzymatic production of vitamin E succinate.

中文翻译：

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有机介质中皱纹念珠菌脂肪酶合成维生素E琥珀酸酯

对用于合成维生素E琥珀酸酯的市售脂肪酶的筛选表明，来自假丝酵母（Candida rugosa）的脂肪酶具有最高的收率。研究了在lgP值介于-1.3至3.5之间的有机溶剂中合成琥珀酸维生素E的过程。特别令人感兴趣的是，在所有使用的有机溶剂中，具有最低IgG的二甲基亚砜（DMSO）表现出最高的收率。由于在有机溶剂中与亲水性和疏水性底物的酶促反应的复杂性，这表明lgP无法令人满意地预测有机溶剂的生物相容性。还研究了不同操作条件的影响，例如底物的摩尔比，酶浓度，反应温度，传质和反应时间。在10 g / L酶的最佳条件下，搅拌速度为100 r / min，底物摩尔比为5：1，在55°C下放置18 h，可获得令人满意的收率（46.95％）。所开发的方法具有用于酶促生产琥珀酸维生素E的潜力。