The pen shell, Pinna nobilis L. is critically endangered by the spread of a haplosporidan parasite. Stable isotopes have been shown an association with dietary assimilation, trophic level, and body condition, and can provide valuable information for ex situ conservation and breeding. In this context, the aim of this study was to investigate the nutritional status of individuals using δ¹³C and δ¹⁵N patterns across pen shell body tissues as tracers of elemental incorporation from treatment diets based on commercial gels and living phytoplankton and/or sediment. Further comparisons were also conducted with field animals and diets to better understand nutritional needs. Captive individuals, were enriched in δ¹³C and δ¹⁵N (~18.9 and 1.3‰, respectively), suggesting severe nutritional stress and utilization of own N and C pools, especially muscle. The mixing model for field individuals evidenced a large contribution from the zooplankton fraction (34.9%), which was further confirmed by experimental feeding with Artemia nauplii.

笔壳，Pinna nobilis L.受到单倍体寄生虫的扩散的严重威胁。稳定同位素已被证明与饮食同化，营养水平和身体状况有关，并且可以为非原位保存和繁殖提供有价值的信息。在此背景下，本研究的目的是调查使用δ个体的营养状况¹³ C和δ ^15种跨笔壳身体组织N种模式从基于商业凝胶和生活浮游植物和/或沉淀物治疗饮食元素掺入的示踪剂。还与野外动物和饮食进行了进一步比较，以更好地了解营养需求。圈养的个体，在δ富集¹³ C和δ ¹⁵氮（分别为〜18.9和1.3‰），表明严重的营养压力以及对氮和碳库（尤其是肌肉）的利用。用于现场个体的混合模型证明从浮游动物分数（34.9％），其进一步通过实验馈送与确认一个大的贡献的卤虫无节幼体。