ABSTRACT

The effect of the brain-derived neurotrophic factor (BDNF), cytokines, and renin angiotensin system (RAS) on memory function have been demonstrated. In this study, the effects of RAS inhibitor captopril (Capto) on hippocampal BDNF, interleukin −6 (IL-6), oxidative stress indicators, and nitric oxide (NO) in scopolamine (Sco)-induced memory impairment in rats were examined. The groups were (1) control, (2) Sco in which Sco was applied 30 min prior to the behavioral tests, and (3–5) Sco-Capto 10, 50, and 100 groups, where Capto (10, 50, or 100 mg/kg), were applied 2 weeks prior to the experiment, as well as 30 min prior to each Sco injection. The Morris Water Maze (MWM) test was conducted, and BDNF, IL-6, NO metabolites, malondialdehyde (MDA), thiol, superoxide dismutase (SOD), and catalase (CAT) were measured. Sco increased the delay and distance to the platform in the MWM test (P < .01 to P < .001), while shortening the time and distance in the target area (P < .01 to P < .001). Additionally, Sco increased IL-6, NO metabolites, and MDA, while decreasing BDNF, thiol, SOD, and CAT (P < .01 to P < .001). Although the Capto reduced the latency and distance traveled to the platform (P < .05 to P < .001), it elevated the time and distance traveled in the target area (P < .05 to P < .01). Furthermore, Capto improved BDNF, thiol, SOD, and CAT levels, and decreased IL-6, NO metabolites, and MDA (P < .05 to P < .001). RAS has a role in learning and memory impairment due to cholinergic system dysfunction. The possible mechanism(s) are including its effects on BDNF, neuro-inflammation and oxidative stress.

摘要

脑源性神经营养因子 (BDNF)、细胞因子和肾素血管紧张素系统 (RAS) 对记忆功能的影响已得到证实。在本研究中，研究了 RAS 抑制剂卡托普利 (Capto) 对东莨菪碱 (Sco) 诱导的大鼠记忆障碍中海马 BDNF、白细胞介素 -6 (IL-6)、氧化应激指标和一氧化氮 (NO) 的影响。各组为 (1) 对照组，(2) Sco，其中在行为测试前 30 分钟应用了 Sco，以及 (3-5) Sco-Capto 10、50 和 100 组，其中 Capto（10、50 或100 mg/kg)，在实验前 2 周以及每次 Sco 注射前 30 分钟使用。进行了莫里斯水迷宫 (MWM) 测试，并测量了 BDNF、IL-6、NO 代谢物、丙二醛 (MDA)、硫醇、超氧化物歧化酶 (SOD) 和过氧化氢酶 (CAT)。P < .01 至P < .001），同时缩短在目标区域的时间和距离（P < .01 至P < .001）。此外，Sco 增加 IL-6、NO 代谢物和 MDA，同时降低 BDNF、硫醇、SOD 和 CAT（P < .01 至P < .001）。尽管 Capto 减少了到达平台的延迟和距离（P < .05 至P < .001），但它增加了在目标区域中行驶的时间和距离（P < .05 至P < .01）。此外，Capto 改善了 BDNF、硫醇、SOD 和 CAT 水平，并降低了 IL-6、NO 代谢物和 MDA（P < .05 至P< .001）。由于胆碱能系统功能障碍，RAS 在学习和记忆障碍中起作用。可能的机制包括其对 BDNF、神经炎症和氧化应激的影响。