Introduction

The use of 3D-bioprinted ovaries has been proven to be a promising technique for preserving fertility. Stereology is an accurate method to obtain quantitative 3D information and the stereological data is the basis for 3D bioprinting ovaries.

Methods

In this study, six female mice were used to acquire the ovarian tissues. One of the two paraffin-embedded ovaries of each mouse was cut into 5 µm sections, and the other was cut into 15 µm sections and then subjected to haematoxylin and eosin staining and anti-follicle stimulating hormone receptor antibody immunohistochemistry. The volume and volume fractions of ovaries were measured by the Cavalieri method. Then, the numerical densities and total numbers of ovarian granulosa cells (OGCs) and primordial, preantral and antral follicles in serial sections were estimated using design-based stereology.

Results

The ovarian volume was 2.50 ± 0.32 mm³. The volume fractions of the cortex, medulla, follicles and OGCs were 86.80% ± 2.82, 13.20% ± 2.82%, 5.60% ± 0.25% and 81.19% ± 2.57%, respectively. The numerical densities of OGCs, the primordial, preantral and antral follicles were 2.11 (± 0.28) × 10⁶/mm³, 719.57 ± 18.04/mm³, 71.84 ± 3.93/mm³ and 17.29 ± 3.54/mm³, respectively. The total number of OGCs and follicles per paraffin-embedded ovary were 5.26 (± 0.09) × 10⁶ and 2013.66 ± 8.16.

Conclusions

The study had obtained the stereological data of the mice ovaries, which contribute to a deeper understanding of the structure of the ovaries. Meanwhile, the data will supply information for 3D bioprinting ovaries.

中文翻译：

---

用于 3D 生物打印应用的小鼠卵巢组织的体视学研究

介绍

使用 3D 生物打印卵巢已被证明是一种很有前途的保持生育能力的技术。体视学是获取定量 3D 信息的准确方法，而体视学数据是 3D 生物打印卵巢的基础。

方法

在这项研究中，六只雌性小鼠被用来获取卵巢组织。每只小鼠的两个石蜡包埋的卵巢中的一个被切成 5μm的切片，另一个被切成15μm的 切片，然后进行苏木精和伊红染色和抗促卵泡激素受体抗体免疫组化。卵巢的体积和体积分数采用卡瓦列里法测量。然后，使用基于设计的体视学估计连续切片中卵巢颗粒细胞 (OGC) 和原始、窦前和窦卵泡的数值密度和总数。

结果

卵巢体积为2.50±0.32 mm ³。皮质、髓质、卵泡和OGC的体积分数分别为86.80%±2.82、13.20%±2.82%、5.60%±0.25%和81.19%±2.57%。OGCs、原始卵泡、窦前卵泡和窦卵泡的数值密度分别为2.11（±0.28）×10 ⁶ /mm ³、719.57±18.04/mm ³、71.84±3.93/mm ³和17.29±3.54/mm ³。每个石蜡包埋卵巢的 OGC 和卵泡总数为 5.26 (± 0.09) × 10 ⁶和 2013.66 ± 8.16。

结论

该研究获得了小鼠卵巢的体视学数据，有助于更深入地了解卵巢的结构。同时，这些数据将为3D生物打印卵巢提供信息。