Abstract

The marine environment envelops an enormous natural assorted diversity and can be considered as an unexplored domain. Diversion of biodiversity is an important part of ensuring the safety and stability of the marine ecosystem. The present study aimed to isolate and characterized the marine halophilic bacteria from the Southeast coast of Tamil Nadu, India. Based on colony morphology the isolated bacteria was characterized by gram staining and biochemical test. The isolated marine halophilic bacteria were screened and identified with a phylogenetic analysis of 16S rRNA gene sequence determined. The result revealed the phylogenetic analysis using 16S rRNA sequence indicated that 6 bacterial isolates belong to Gram-positive phylum of Firmicutes (CARE-V2 Bacillus flexus, CARE-V12 Virgibacillus halodenitrificans, CARE-V17 Virgibacillus salaries, CARE-V29 Jeotgalicoccus huakuii, CARE-V33 Oceanobacillus iheyensis, and CARE-V34 Virgibacillus sp), and 6 bacterial strains accord to Gram-negative phylum of proteobacteria (CARE-V3 Halomonas sp, CARE-V5 Oceanimonas sp, CARE-V9 Bacterium, CARE-V10 Halomonas salifodinae, CARE-V14 Halomonas venusta, CARE-V31 Salinivibrio sp). This study summarized the marine halophilic bacteria and the diversity of marine microorganisms from the Southeast coast of Tamil Nadu.

摘要

海洋环境包含着巨大的自然多样性，可以被视为一个未开发的领域。生物多样性的转移是确保海洋生态系统安全稳定的重要组成部分。本研究旨在分离和表征来自印度泰米尔纳德邦东南海岸的海洋嗜盐细菌。基于菌落形态，分离的细菌通过革兰氏染色和生化测试表征。对分离的海洋嗜盐细菌进行了筛选和鉴定，并通过确定的 16S rRNA 基因序列的系统发育分析进行了鉴定。结果显示，使用16S rRNA序列进行的系统发育分析表明，6株细菌分离株属于厚壁菌门革兰氏阳性门（CARE-V2 Bacillus flexus， CARE-V12Virgibacillus halodenitrificans， CARE-V17 Virgibacillus工资， CARE-V29 Jeotgalicoccus huakuii， CARE-V33 Oceanobacillus iheyensis，和CARE-V34 Virgibacillus SP），和6个菌株符合于变形菌的革兰氏阴性门（CARE-V3盐单胞菌属，转交V5 Oceanimonas sp、CARE-V9细菌、 CARE-V10 Halomonas salifodinae、 CARE-V14 Halomonas venusta、 CARE-V31 Salinivibrio sp)。本研究总结了泰米尔纳德邦东南海岸的海洋嗜盐细菌和海洋微生物的多样性。