Biological technology has presented a promising application for phosphine (PH₃) purification; however, the degradation characteristics and microbial community of PH₃ biopurification systems have rarely been reported. This study introduced an activated sludge process to treat PH₃ in off-gas. Besides PH₃ removal, oxidase activity and other characteristics such as phosphorous transformation and microbial community were investigated. When the PH₃ concentration of the inlet gas was <30 mg/m³, the bioreactor achieved a relatively higher rate of PH₃ removal, with a maximum rate of 72.9%. The activity of the enzyme superoxide dismutase increased with PH₃ concentration; however, the bioreactor with an inlet gas PH₃ concentration of 45 mg/m³ maintained lower catalase activity, revealing that high PH₃ concentration had an adverse effect on biological process owing to PH₃-induced oxidizing stress. Gaseous PH₃ can be adsorbed by microbial organisms, but its final removal depends on microbial metabolism. Phosphorus converted from PH₃ was eventually assimilated by microbes, and the total phosphorus in the supernatant was usually <1.0 mg/L during the whole operation period. Proteobacteria and Firmicutes were two major phyla in PH₃ biopurification systems, and the genera Burkholderia and Methylophilus developed into the dominant microbes in activated sludge bioreactors, and these complex microbial communities may facilitate PH₃ biopurification.

中文翻译：

---

磷化氢生物净化系统的降解特性及微生物群落

生物技术为磷化氢 (PH ₃ ) 纯化提供了一个有前景的应用；然而，PH ₃生物净化系统的降解特性和微生物群落却鲜有报道。本研究介绍了一种活性污泥法来处理废气中的PH ₃。除了去除PH ₃外，还研究了氧化酶活性和其他特征，如磷转化和微生物群落。当入口气体的PH ₃浓度<30 mg/m ^{3 时}，生物反应器的PH ₃去除率相对较高，最高可达72.9%。酶超氧化物歧化酶的活性随PH升高₃浓度；然而，入口气体PH ₃浓度为45 mg/m ³的生物反应器保持较低的过氧化氢酶活性，表明由于PH ₃诱导的氧化应激，高PH ₃浓度对生物过程具有不利影响。气态PH ₃可被微生物吸附，但其最终去除取决于微生物代谢。PH ₃转化的磷最终被微生物同化，整个操作期间上清液中的总磷通常<1.0 mg/L。Proteobacteria 和 Firmicutes 是 PH ₃生物纯化系统中的两个主要门，伯克霍尔德菌属和Methylophilus发展成为活性污泥生物反应器中的主要微生物，这些复杂的微生物群落可能促进 PH ₃生物净化。