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Antimycotic effects of a prodigiosin producing Serratia marcescens rhizobacteria
Rhizosphere ( IF 3.4 ) Pub Date : 2021-03-16 , DOI: 10.1016/j.rhisph.2021.100336
C. Jimtha John , A.V. Bibishna , G.E. Mallikarjunaswamy

Pigmented Serratia spp. have antibacterial, antimalarial, antimycotic, immunomodulatory and antitumor activities. Here, a pigmented Rhizobacteria, Serratia spp. isolated from medicinal plant Plumbago indica was studied for its inhibitory effect towards Pythium myriotylum. Upon microscopic analysis, the pigment extract prepared from isolated Serratia spp. was found to cause remarkable distortion on fungal morphology exhibiting an inhibition of 40%. Ginger rhizomes stand uninfected with this extract treatment similar to that of rhizome treated with commercial biocontrol agent Delfin (R), a Bacillus thuringiensis product. For structural characterization, the water-insoluble red pigment was extracted using acidified ethanol. This was followed by analysis of thin layer chromatography (TLC), UV-Visible Spectrometry showing an absorbance maxima at 535 nm, FTIR and GC-MS revealing mass-to-charge ratio (m/z) of 310 [M + H]+ that confirmed the prodigiosin pigment. Therefore, the broad antifungal activity of the prodigiosin-producing strain indicates its potential to be exploited as a natural ecofriendly biocontrol agent.



中文翻译:

产生prodigiosin的粘质沙雷氏菌根瘤菌的抗真菌作用

色素沙雷菌属具有抗菌,抗疟疾,抗真菌,免疫调节和抗肿瘤活性。在这里,有色的根瘤菌,沙雷氏菌。研究了从药用植物东北铅( Plumbago indica)分离的大豆腐霉病的抑制作用。经显微镜分析,从分离的沙雷氏菌属Serratia spp)制备色素提取物。据发现,其引起真菌形态的显着畸变,表现出40%的抑制作用。姜根茎未受到这种提取物处理的感染,类似于用商业生物防治剂Delfin(R)(苏云金芽孢杆菌)处理过的根茎。产品。为了进行结构表征,使用酸化的乙醇提取水不溶性红色颜料。随后进行薄层色谱分析(TLC),紫外可见光谱显示535 nm处的最大吸光度,FTIR和GC-MS揭示质荷比(m / z)为310 [M + H] +证实了prodigiosin色素。因此,产生前靛红素的菌株的广泛的抗真菌活性表明其被开发为天然的生态友好型生物防治剂的潜力。

更新日期:2021-03-30
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