We evaluated how genotype (stock source, parental line, chromosomal sex) and temperature interact to influence gonad development and phenotypic sex of Oncorhynchus mykiss. Embryos were generated from single pair matings of natural-origin and first-generation hatchery-origin O. mykiss held at ambient or elevated (5 °C above ambient) water temperature. Exposure to elevated temperature lasted from fertilization until the onset of exogenous feeding, after which all experimental groups were maintained at ambient temperature. Sexual phenotype was determined by histology and sexual genotype by the OmyY1 marker. There was 99% concordance among fish sexed both phenotypically and genotypically, demonstrating that experimental factors, including elevated rearing temperatures, did not result in sex change. Approximately 10% of the histologically examined fish showed no evidence of gonad development beyond the indifferent stage. Most of these fish were from hatchery family lines.

我们评估了基因型（种群来源，亲本系，染色体性别）和温度如何相互作用，以影响Oncorhynchus mykiss的性腺发育和表型性别。胚胎是由天然起源的卵和第一代孵化场起源的O. mykiss的单对交配产生的保持在环境温度或升高的温度（高于环境温度5°C）下。从受精到暴露于升高的温度一直持续到外源进食开始，此后所有实验组均保持在环境温度下。性表型由组织学确定，性基因型由OmyY1标记确定。在表型和基因型性别上的鱼中，有99％的一致性，表明包括升高的饲养温度在内的实验因素并未导致性别变化。经组织学检查的鱼类中，大约有10％的人在无动于衷的阶段之后都没有性腺发育的迹象。这些鱼大多数来自孵化场的家系。