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Temporal recovery of soil biological activity and diversity after 915-MHz microwave treatments
Biology and Fertility of Soils ( IF 6.5 ) Pub Date : 2021-03-16 , DOI: 10.1007/s00374-021-01553-5
Géraldine Maynaud , Ezékiel Baudoin , Frédéric Mahé , Cécile Villenave , Jean-Claude Cleyet-Marel , Brigitte Brunel

Two 915-MHz microwave treatments (2 kW-8 min and 4 kW-4 min) were applied to soil and their effects were monitored just after treatments (T0) and 26 days (T26) in soil microcosms. Densities of culturable bacteria, fluorescent pseudomonads and nematodes, hydrolysis activity and soil DNA content declined by over 50% immediately after both microwave treatments (T0), excluding the total fungal 18S rRNA (−13 or −17%) and bacterial 16S rRNA copies (non-significant). A rapid shift in bacterial community composition occurred from T0 towards a large increase in the relative abundance of Firmicutes (+1650%) and a concomitant decrease in various phyla (e.g. Acidobacteria, Actinobacteria, Bacteroidetes, Proteobacteria) from −85 to −61%. At T26 and for both treatments, fluorescein diacetate hydrolysis, density of culturable bacteria, 18S rRNA gene numbers, Simpson diversity, relative abundances of Bacteroidetes and Proteobacteria regained levels similar to controls. Alpha, Delta and Gamma classes Proteobacteria also end up reaching a similar level. In contrast, fluorescent pseudomonad density, nematode diversity and abundance, soil DNA content and relative abundances of some phylum (Acidobacteria, Actinobacteria, Chloroflexi) did not increase in such proportions. Most of the soil biological properties have not been permanently impacted. Nevertheless, the recovery kinetics highly differed according properties, with resilience indices at T26 varying from −87 to +99%.



中文翻译:

915 MHz微波处理后土壤生物活性和多样性的时间恢复

对土壤进行了两次915 MHz微波处理(2 kW-8分钟和4 kW-4分钟),并在土壤微观世界中分别在处理(T0)和26天(T26)之后监测了它们的效果。两种微波处理(T0)后,可培养细菌,荧光假单胞菌和线虫的密度,水解活性和土壤DNA含量均立即下降了50%以上,但不包括真菌18S rRNA的总含量(−13或-17%)和细菌16S rRNA的含量(不重要)。细菌群落组成发生了快速变化,从T0到Firmicutes的相对丰度大幅增加(+ 1650%),各种门(例如,酸性细菌,放线菌,拟杆菌,变形杆菌)的减少也从-85%降低到-61%。在T26和两种处理中,荧光素双乙酸酯水解,可培养细菌的密度,18S rRNA基因编号,辛普森多样性,拟杆菌和变形杆菌的相对丰度恢复到与对照相似的水平。阿尔法,德尔塔和伽玛类变形杆菌也最终达到了类似的水平。相反,荧光假单胞菌的密度,线虫的多样性和丰度,土壤DNA含量以及某些门(Acidobacteria,Actinobacteria,Chloroflexi)的相对丰度并未以这种比例增加。大多数土壤生物学特性尚未受到永久性影响。然而,恢复动力学根据性质而有很大差异,在T26时的回弹指数从-87%到+ 99%不等。线虫的多样性和丰度,某些门(Acidobacteria,Actinobacteria,Chloroflexi)的土壤DNA含量和相对丰度没有以这种比例增加。大多数土壤生物学特性尚未受到永久性影响。然而,恢复动力学根据性质而有很大差异,在T26时的回弹指数从-87%到+ 99%不等。线虫的多样性和丰度,某些门(Acidobacteria,Actinobacteria,Chloroflexi)的土壤DNA含量和相对丰度没有以这种比例增加。大多数土壤生物学特性尚未受到永久性影响。然而,恢复动力学根据性质而有很大差异,在T26时的回弹指数从-87%到+ 99%不等。

更新日期:2021-03-16
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