Steam explosion (SE) pretreatment is a widely adopted method for enhancing enzymatic hydrolysis of lignocellulosic biomass in biorefinery. In this work, moderate SE pretreatment was employed to overcome the recalcitrance of cell wall through systematically assessing physicochemical and structural modifications. The hydrolysis yield of pretreated poplar by SE pretreatment (up to 69.7%) was higher than untreated poplar (26.8%). With increasing SE severity from 3.5 to 4.5, the contents of hemicellulose and lignin were reduced from 15.7 to 5.3% and 25.4 to 20.7%, respectively. Furthermore, it was observed that the cell wall structure became rough and collapsed. Meanwhile, channels emerged in the internal cell wall primarily due to the removal of hemicellulose and lignin along with the migration of lignin from inside to the out surface of cell wall. These results induced more exposure of cellulose to enzyme attacking, thus improved accessibility to cellulolytic enzymes. Addition of bovine serum albumin (BSA) obtained equal hydrolysis efficiency of 70.1% with a 33% reduction of enzyme loading, which was advantageous for enzyme costs effective.

蒸汽爆炸（SE）预处理是在生物精炼厂中增强木质纤维素生物质酶促水解的一种广泛采用的方法。在这项工作中，通过系统地评估理化和结构修饰，采用适度的SE预处理来克服细胞壁的顽固性。SE预处理对杨树的水解得率（高达69.7％）高于未处理杨树（26.8％）。随着SE严重程度从3.5增加到4.5，半纤维素和木质素的含量分别从15.7％降低到5.3％和25.4％降低到20.7％。此外，观察到细胞壁结构变得粗糙并且塌陷。同时，主要由于半纤维素和木质素的去除以及木质素从细胞壁的内部向外部的迁移，通道在内部细胞壁中出现。这些结果引起纤维素更多地暴露于酶攻击，从而改善了对纤维素分解酶的可及性。加入牛血清白蛋白（BSA）可获得70.1％的同等水解效率，同时酶的负载减少了33％，这对于有效的酶成本而言是有利的。