Nanoparticles (25–50 nm) of chemically modified calcium phosphates Ca 10− x − y M ii x Na y (PO 4 ) 6− z (CO 3 ) z (OH) 2 (M ii – Cu 2+ , Zn 2+ ) were synthesized via a wet precipitation method at room temperature. The Fourier-transform infrared spectroscopy data confirmed the partial substitution of PO43−{\text{PO}}_{4}^{3-} → CO32−{\text{CO}}_{3}^{2-} (B-type) in apatite-type structure. The influence of prepared phosphates on biofilm formation by pathogenic microorganisms was investigated. It was found that the samples Na + , CO32−{\text{CO}}_{3}^{2-}-hydroxyapatite (HAP) and Na + , Zn 2+ , CO32−{\text{CO}}_{3}^{2-}-HAP (5–20 mM) had the highest inhibitory effect on biofilm formation by Staphylococcus aureus strains. The sample Na + , CO32−{\text{CO}}_{3}^{2-}-HAP had the slight influence on the formation of the biofilm by Pseudomonas aeruginosa , while for the samples Na + , Cu 2+ , CO32−{\text{CO}}_{3}^{2-}-HAP and Na + , Zn 2+ , CO32−{\text{CO}}_{3}^{2-}-HAP such an effect was not detected. According to transmission electron microscopy data, a correlation between the activity of synthesized apatite-related modified calcium phosphates in the processes of biofilm formation and their ability to adhere to the surface of bacterial cells was established. The prepared samples can be used for the design of effective materials with antibacterial activity for medicine.

中文翻译：

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纳米改性磷灰石型磷酸钙对病原微生物生物膜形成的影响

化学修饰的磷酸钙的纳米粒子（25–50 nm）Ca 10− x − y M ii x Na y（PO 4）6−z（CO 3）z（OH）2（M ii – Cu 2+，Zn 2+通过湿沉淀法在室温下合成）。傅立叶变换红外光谱数据证实了PO43-{\ text {PO}} _ {4} ^ {3-}→CO32-{\ text {CO}} _ {3} ^ {2-}（ B型）中的磷灰石型结构。研究了制备的磷酸盐对病原微生物形成生物膜的影响。发现样品Na +，CO32 − {\ text {CO}} __ 3 ^ {2-}-羟基磷灰石（HAP）和Na + Zn 2+，CO32 − {\ text {CO}} _ {3} ^ {2-}-HAP（5–20 mM）对金黄色葡萄球菌菌株对生物膜形成的抑制作用最高。样品Na +，CO32-{\ text {CO}} _ {3} ^ {2-}-HAP对铜绿假单胞菌的生物膜形成影响很小，而Na +，Cu 2+，CO32-{\ text {CO}} _ {3} ^ {2-}-HAP和Na +，Zn 2+，CO32 − {\ text {CO}} _ {3} ^ {2-}-HAP和HAP均未检测到这种效果。根据透射电子显微镜数据，在生物膜形成过程中合成的磷灰石相关的改性磷酸钙的活性与其粘附在细菌细胞表面的能力之间建立了相关性。所制备的样品可用于设计具有抗菌活性的有效材料。建立了在生物膜形成过程中合成的磷灰石相关的改性磷酸钙的活性与其粘附在细菌细胞表面的能力之间的相关性。制备的样品可用于设计具有抗菌活性的有效材料。建立了在生物膜形成过程中合成的磷灰石相关的改性磷酸钙的活性与其粘附在细菌细胞表面的能力之间的相关性。制备的样品可用于设计具有抗菌活性的有效材料。