This study aimed to identify the effectiveness of SNEDDS of Pegagan Leaf Ethanol Extract (PLE) to reduce fasting blood glucose (FBG) levels in zebrafish. Centella asiatica (L.) Urb. or pegagan is among the medicinal plants widely used to treat diabetes in Indonesia. Maceration was employed with 70% ethanol to obtain a viscous extract for the formulation of SNEDDS with Capryol 90, Tween 80, and PEG 400 (1:6:3). Antihyperglycemic testing was conducted on five groups, consisting of normal, positive control, negative control, P I treatment, and P II treatment. On Day 1, all except the normal group was induced with 300 mg alloxan and soaked in 2% glucose solution for 7 days. On day 8, the treatment consisted of 25 mg/2 L metformin for the positive control, 100 mg/2 L SNEDDS for P I, 200 mg/2 L SNEDDS for P II, and no treatment for the negative control. The SNEDDS characterization obtained 100.6 ± 3.12 nm particle size and −7.93 ± 0.66 mV zeta potential, indicating that the SNEDDS had fulfilled the requirements of good preparation. The antidiabetic activity test found a 69.90% decline in FBG levels in 100 mg/2 L SNEDDS and 72.20% in 200 mg/2 L SNEDDS.

中文翻译：

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积雪草（L.）Urb的降血糖活性。斑马鱼中的叶片乙醇提取物SNEDDS（Danio rerio）

这项研究旨在确定七叶树叶乙醇提取物（PLE）的SNEDDS降低斑马鱼空腹血糖（FBG）水平的有效性。积雪草（L.）Urb。在印度尼西亚，pegagan或pegagan是广泛用于治疗糖尿病的药用植物之一。将浸渍液与70％乙醇一起使用，以获得粘性提取物，用于与Capryol 90，Tween 80和PEG 400（1：6：3）配制成SNEDDS。对五个组进行了降血糖测试，包括正常，阳性对照，阴性对照，PI治疗和P II治疗。在第1天，用300mg四氧嘧啶诱导除正常组外的所有动物，并在2％葡萄糖溶液中浸泡7天。在第8天，治疗包括：25 mg / 2 L二甲双胍作为阳性对照，100 mg / 2 L SNEDDS用于PI，200 mg / 2 L SNEDDS用于P II，而阴性对照则不进行治疗。SNEDDS表征获得100.6±3.12 nm粒径和-7.93±0.66 mV zeta电位，表明SNEDDS已满足良好制备的要求。抗糖尿病活性测试发现100 mg / 2 L SNEDDS中FBG水平下降69.90％，而200 mg / 2 L SNEDDS中FBG水平下降72.20％。