The interpeduncular nucleus (IPN) is a highly conserved limbic structure in the vertebrate brain, located in the isthmus and rhombomere 1. It is formed by various populations that migrate from different sites to the distinct domains within the IPN: the prodromal, rostral interpeduncular, and caudal interpeduncular nuclei. The aim here was to identify genes that are differentially expressed across these domains, characterizing their putative functional roles and interactions. To this end, we screened the 2038 genes in the Allen Developing Mouse Brain Atlas database expressed at E18.5 and we identified 135 genes expressed within the IPN. The functional analysis of these genes highlighted an overrepresentation of gene families related to neuron development, cell morphogenesis and axon guidance. The interactome analysis within each IPN domain yielded specific networks that mainly involve members of the ephrin/Eph and Cadherin families, transcription factors and molecules related to synaptic neurotransmission. These results bring to light specific mechanisms that might participate in the formation, molecular regionalization, axon guidance and connectivity of the different IPN domains. This genoarchitectonic model of the IPN enables data on gene expression and interactions to be integrated and interpreted, providing a basis for the further study of the connectivity and function of this poorly understood nuclear complex under both normal and pathological conditions.

中文翻译：

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足突间核中的多个区域化基因及其推定网络表明神经元发育和轴突引导的复杂机制。

足突间核（IPN）是脊椎动物脑中高度保守的边缘结构，位于峡部和菱形1中。它是由从不同部位迁移到IPN内不同区域的各种种群形成的：前额，延髓鼻端，和尾椎间核。这里的目的是鉴定在这些结构域中差异表达的基因，表征其假定的功能角色和相互作用。为此，我们在艾伦发展小鼠脑图集数据库中筛选了以E18.5表达的2038个基因，并鉴定了在IPN中表达的135个基因。这些基因的功能分析突出显示了与神经元发育，细胞形态发生和轴突导向相关的基因家族的过度表达。每个IPN域中的相互作用组分析产生了特定的网络，该网络主要涉及ephrin / Eph和Cadherin家族成员，转录因子和与突触神经传递有关的分子。这些结果揭示了可能参与不同IPN域的形成，分子区域化，轴突引导和连通性的特定机制。IPN的这种基因构造模型使基因表达和相互作用的数据得以整合和解释，从而为进一步研究这种知之甚少的核复合体在正常和病理条件下的连通性和功能提供了基础。这些结果揭示了可能参与不同IPN域的形成，分子区域化，轴突引导和连通性的特定机制。IPN的这种基因构造模型使基因表达和相互作用的数据得以整合和解释，从而为进一步研究这种知之甚少的核复合体在正常和病理条件下的连通性和功能提供了基础。这些结果揭示了可能参与不同IPN域的形成，分子区域化，轴突引导和连通性的特定机制。IPN的这种基因构造模型使基因表达和相互作用的数据得以整合和解释，从而为进一步研究这种知之甚少的核复合体在正常和病理条件下的连通性和功能提供了基础。