Protein profiles that can predict allergy development in children are lacking and the ideal sampling age is unknown. By applying an exploratory proteomics approach in the prospective FARMFLORA birth cohort, we sought to identify previously unknown circulating proteins in early life that associate to protection or risk for development of allergy up to 8 years of age. We analyzed plasma prepared from umbilical cord blood (n = 38) and blood collected at 1 month (n = 42), 4 months (n = 39), 18 months (n = 42), 36 months (n = 42) and 8 years (n = 44) of age. We profiled 230 proteins with a multiplexed assay and evaluated the global structure of the data with principal component analysis (PCA). Protein profiles informative to allergic disease at 18 months, 36 months and/or 8 years were evaluated using Lasso logistic regression and random forest. Two clusters emerged in the PCA analysis that separated samples obtained at birth and at 1 month of age from samples obtained later. Differences between the clusters were mostly driven by abundant plasma proteins. For the prediction of allergy, both Lasso logistic regression and random forest were most informative with samples collected at 1 month of age. A Lasso model with 27 proteins together with farm environment differentiated children who remained healthy from those developing allergy. This protein panel was primarily composed of antigen-presenting MHC class I molecules, interleukins and chemokines. Sampled at one month of age, circulating proteins that reflect processes of the immune system may predict the development of allergic disease later in childhood.

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与婴儿过敏发展相关的循环蛋白-探索性分析

尚缺乏可预测儿童过敏发展的蛋白质谱，理想的采样年龄尚不清楚。通过在前瞻性FARMFLORA出生队列中应用探索性蛋白质组学方法，我们试图鉴定早年未知的循环蛋白，这些蛋白与长达8岁的保护或发生过敏的风险相关。我们分析了从脐带血（n = 38）和在1个月（n = 42），4个月（n = 39），18个月（n = 42），36个月（n = 42）和8时采集的血液制备的血浆岁（n = 44）岁。我们使用多重分析对230种蛋白质进行了分析，并使用主成分分析（PCA）评估了数据的整体结构。使用Lasso logistic回归和随机森林评估了18个月，36个月和/或8年时对过敏性疾病有益的蛋白质谱。PCA分析中出现了两个聚类，它们将出生时和1个月大时获得的样品与后来获得的样品分开。簇之间的差异主要是由丰富的血浆蛋白驱动的。对于变态反应的预测，Lasso logistic回归和随机森林对1个月大时收集的样本信息最为丰富。具有27种蛋白质的Lasso模型以及农场环境使仍健康的儿童与发生过敏的儿童区分开。该蛋白质组主要由呈递抗原的MHC I类分子，白介素和趋化因子组成。在一个月大的时候取样，反映免疫系统过程的循环蛋白可能预示着儿童期以后过敏性疾病的发展。