Dermal injury, including trauma, surgical incisions, and burns, remain the most prevalent socio-economical health care issue in the clinic. Nanomedicine represents a reliable administration strategy that can promote the healing of skin lesions, but the lack of effective drug delivery methods can limit its effectiveness. In this study, we developed a novel nano-drug delivery system to treat skin defects through spraying. We prepared curcumin-loaded chitosan nanoparticles modified with epidermal growth factor (EGF) to develop an aqueous EGF-modified spray (EGF@CCN) for the treatment of dermal wounds. In vitro assays showed that the EGF@CCN displayed low cytotoxicity, and that curcumin was continuously and slowly released from the EGF@CCN. In vivo efficacy on wound healing was then evaluated using full-thickness dermal defect models in Wistar rats, showing that the EGF@CCN had significant advantages in promoting wound healing. On day 12 post-operation, skin defects in the rats of the EGF@CCN group were almost completely restored. These effects were related to the activity of curcumin and EGF on skin healing, and the high compatibility of the nano formulation. We therefore conclude that the prepared nano-scaled EGF@CCN spray represents a promising strategy for the treatment of dermal wounds.

中文翻译：

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EGF修饰姜黄素/壳聚糖纳米喷雾剂对伤口愈合的治疗作用

皮肤损伤，包括创伤、手术切口和烧伤，仍然是临床上最普遍的社会经济医疗保健问题。纳米医学代表了一种可靠的给药策略，可以促进皮肤病变的愈合，但缺乏有效的药物输送方法可能会限制其有效性。在这项研究中，我们开发了一种新型纳米药物输送系统，通过喷雾治疗皮肤缺陷。我们制备了表皮生长因子（EGF）修饰的姜黄素负载壳聚糖纳米颗粒，开发了一种水性EGF修饰喷雾剂（EGF@CCN），用于治疗真皮伤口。体外试验表明，EGF@CCN 显示出较低的细胞毒性，并且姜黄素从 EGF@CCN 中持续缓慢地释放。然后使用 Wistar 大鼠的全层真皮缺损模型评估了伤口愈合的体内功效，表明 EGF@CCN 在促进伤口愈合方面具有显着优势。术后第12天，EGF@CCN组大鼠皮肤缺损几乎完全恢复。这些效果与姜黄素和EGF对皮肤愈合的活性以及纳米配方的高相容性有关。因此，我们得出的结论是，制备的纳米级 EGF@CCN 喷雾剂代表了治疗真皮伤口的一种有前途的策略。