Reactive oxygen species (ROS) are byproducts of cellular metabolism; they play a significant role as secondary messengers in cell signaling. In cells, high concentrations of ROS induce apoptosis, senescence, and contact inhibition, while low concentrations of ROS result in angiogenesis, proliferation, and cytoskeleton remodeling. Thus, controlling ROS generation is an important factor in cell biology. We designed a chlorin e6 (Ce6)-immobilized polyethylene terephthalate (PET) film (Ce6-PET) to produce extracellular ROS under red-light irradiation. The application of Ce6-PET films can regulate the generation of ROS by altering the intensity of light-emitting diode sources. We confirmed that the Ce6-PET film could effectively promote cell growth under irradiation at 500 μW/cm2 for 30 min in human umbilical vein endothelial cells. We also found that the Ce6-PET film is more efficient in generating ROS than a Ce6-incorporated polyurethane film under the same conditions. Ce6-PET fabrication shows promise for improving the localized delivery of extracellular ROS and regulating ROS formation through the optimization of irradiation intensity.

中文翻译：

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使用二氢卟酚 e6 固定 PET 膜作为光功能生物材料产生可控水平的 ROS 以增强 HUVEC 增殖的有效方法


活性氧 (ROS) 是细胞代谢的副产品；它们作为细胞信号转导中的第二信使发挥着重要作用。在细胞中，高浓度的 ROS 会诱导细胞凋亡、衰老和接触抑制，而低浓度的 ROS 会导致血管生成、增殖和细胞骨架重塑。因此，控制ROS的产生是细胞生物学的一个重要因素。我们设计了一种二氢卟酚e6（Ce6）固定的聚对苯二甲酸乙二醇酯（PET）薄膜（Ce6-PET），可在红光照射下产生细胞外ROS。 Ce6-PET薄膜的应用可以通过改变发光二极管光源的强度来调节ROS的产生。我们证实Ce6-PET薄膜在500 μW/cm2照射30分钟下可以有效促进人脐静脉内皮细胞的细胞生长。我们还发现，在相同条件下，Ce6-PET 薄膜比掺有 Ce6 的聚氨酯薄膜更能有效地产生 ROS。 Ce6-PET 制造有望通过优化照射强度来改善细胞外 ROS 的局部递送并调节 ROS 的形成。