Decellularization method based on trypsin-digestion is widely used to construct small diameter vascular grafts. However, this method will reduce the opening angle of the blood vessel and result in the reduction of residual stress. Residual stress reduced has an adverse effect on the compliance and permeability of small diameter vascular grafts. To improve the situation, acellular blood vessels were treated with glutaraldehyde and photooxidation crosslinking respectively, and the changes of opening angle, circumferential residual strain of native blood vessels, decellularized arteries and crosslinked blood vessels were measured by means of histological examination, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) in this study. The opening angle of decellularized arteries significantly restored after photooxidation crosslinking (P = 0.0216), while that of glutaraldehyde crosslinking blood vessels reduced. The elastic fibers inside the blood vessels became densely rearranged after photooxidation crosslinking. The results of finite element simulation showed that the residual stress increased with the increase of opening angle. In this study, we found at the first time that photooxidation crosslinking method could significantly increase the residual stress of decellularized vessels, which provides biomechanical support for the development of new biomaterials of vascular grafts.

中文翻译：

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光氧化交联恢复脱细胞血管中的残余应力


基于胰蛋白酶消化的脱细胞方法被广泛用于构建小直径血管移植物。但这种方法会减小血管的张开角度，导致残余应力减少。残余应力的降低对小直径血管移植物的顺应性和渗透性有不利影响。为了改善这种情况，分别采用戊二醛和光氧化交联处理脱细胞血管，并通过组织学检查、扫描电镜等方法测量天然血管、脱细胞动脉和交联血管的张开角度、周向残余应变的变化。本研究中的 SEM）和透射电子显微镜（TEM）。光氧化交联后脱细胞动脉的张角显着恢复（P = 0.0216），而戊二醛交联血管的张角则减小。光氧化交联后，血管内的弹性纤维变得密集地重新排列。有限元模拟结果表明，残余应力随着张开角度的增大而增大。本研究首次发现光氧化交联方法能够显着增加脱细胞血管的残余应力，为新型血管移植生物材料的开发提供了生物力学支持。