ABSTRACT

Isotope fractionation of fatty acids can reflect the metabolic pathway of fatty acids in organisms, but few studies have been conducted. In this study, stable carbon isotope fractionation of fatty acids in sea cucumber (Apostichopus japonicus) was conducted by feeding experiments of comparing the fatty acid profile and δ¹³C of fatty acids in body wall to diet. The results showed that the fractionation of δ¹³C of fatty acids in A. japonicus was −1.588–7.403‰. The fractionation of δ¹³C of saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), and polyunsaturated fatty acids (PUFAs) was 1.098–7.403‰, −1.588–2.778‰, and −1.559–5.614‰, respectively. The degree of fractionation of δ¹³C of SFAs was greater than that of unsaturated fatty acids (UFAs). We simulated the possible metabolic process of fatty acids in A. japonicus based on fatty acid profile and δ¹³C of fatty acids, indicating that the combination of these two methods could be potentially used in the study of fatty acid metabolism in marine benthic animal of A. japonicus. Our finding provides a new technique for the metabolic pathways of biomolecules in organisms.

摘要

脂肪酸的同位素分馏可以反映生物体内脂肪酸的代谢途径，但鲜有研究。本研究通过比较体壁脂肪酸谱和脂肪酸δ ¹³ C与日粮的饲喂实验，对海参（Apostichopus japonicus ）中的脂肪酸进行稳定碳同位素分离。结果表明，刺参中脂肪酸δ ¹³ C的分馏为-1.588~7.403‰。饱和脂肪酸（SFA）、单不饱和脂肪酸（MUFA）和多不饱和脂肪酸（PUFA）的δ ^{13 C分馏分别为1.098-7.403‰、-1.588-2.778‰和-1.559-5.614‰。δ 13}的分馏程度SFA 的 C 高于不饱和脂肪酸 (UFA)。我们基于脂肪酸谱和脂肪酸的δ ¹³ C模拟了刺参中脂肪酸可能的代谢过程，表明这两种方法的结合可以潜在地用于海洋底栖动物脂肪酸代谢的研究。刺参。我们的发现为生物体中生物分子的代谢途径提供了一种新技术。