Characterization and functional analysis of a β‐adrenergic‐like octopamine receptor from the oriental armyworm (Mythimna separata Walker),Archives of Insect Biochemistry and Physiology

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Characterization and functional analysis of a β‐adrenergic‐like octopamine receptor from the oriental armyworm (Mythimna separata Walker)
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-03-15 , DOI: 10.1002/arch.21772
Yan‐fang Guo ₁ , Jia‐ren Qiu ₁ , Tao Chen ₁ , San‐ji Gao ₁ , Bu Su‐hong ₁ , Ran Wang ₂ , Jin‐da Wang ₁

Affiliation

The β‐adrenergic‐like octopamine receptor (OA2B2), which binds the biogenic amine octopamine, belongs to the class of G‐protein coupled receptors and significantly regulates many physiological and behavioral processes in insects. In this study, the putative open reading frame sequence of the MsOA2B2 gene in Mythimna separata was cloned, the full‐length complementary DNA was 1191 bp and it encoded a 396‐amino acid protein (GenBank accession number MN822800). Orthologous sequence alignment, phylogenetic tree analysis, and protein sequence analysis all showed that the cloned receptor belongs to the OA2B2 protein family. Real‐time quantitative polymerase chain reaction of spatial and temporal expression analysis revealed that the MsOAB2 gene was expressed in all developmental stages of M. separata and was most abundant in egg stages and second and fourth instars compared with other developmental stages, while the expression level during the pupal stage was much lower than that at the other stages. Further analysis with sixth instar M. separata larvae showed that the MsOA2B2 gene was expressed 1.81 times higher in the head than in integument and gut tissues. Dietary ingestion of dsMsOA2B2 significantly reduced the messenger RNA level of MsOA2B2 and decreased mortality following amitraz treatment. This study provides both a pharmacological characterization and the gene expression patterns of OA2B2 in M. separata, facilitating further research for insecticides using MsOA2B2 as a target.

中文翻译：

东方粘虫（Mythimna separata Walker）的β-肾上腺素样章鱼胺受体的表征和功能分析

β-肾上腺素能类章鱼胺受体（OA2B2）与生物胺类章鱼胺结合，属于G蛋白偶联受体，可显着调节昆虫的许多生理和行为过程。在这项研究中，克隆了Mythimna separata中MsOA2B2基因的推定开放阅读框序列，全长互补DNA为1191 bp，编码一个396个氨基酸的蛋白质（GenBank登录号MN822800）。直系同源序列比对，系统发育树分析和蛋白质序列分析均显示克隆的受体属于OA2B2蛋白质家族。实时定量聚合酶链反应的时空表达分析表明，MsOAB2该基因在分离支原体的所有发育阶段均有表达，与其他发育阶段相比，在卵期，第二和第四龄期最丰富，而the阶段的表达水平则远低于其他阶段。对第六龄幼虫分离支原体幼虫的进一步分析表明，MsOA2B2基因在头部的表达量是其外皮和肠组织的1.81倍。饮食中摄入dsMsOA2B2可以显着降低MsOA2B2的信使RNA水平，并降低阿米特拉治疗后的死亡率。这项研究同时提供了药理学表征和在OA2B2的基因表达模式粘虫，便于使用杀虫剂进一步研究以MsOA2B2为目标。

更新日期：2021-04-01

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