Stimulated emission depletion microscopy for biological imaging in four dimensions: A review,Microscopy Research and Technique

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Stimulated emission depletion microscopy for biological imaging in four dimensions: A review
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2021-03-13 , DOI: 10.1002/jemt.23750
Zhaoyang Wu ₁ , Xinzhu Xu ₁ , Peng Xi _{1,

2}

Affiliation

Stimulated emission depletion (STED) microscopy allows high lateral and axial resolution, long term imaging in living cells. Here we review recent technical advances in STED microscopy, with emphasis on resolution and measurement range of XYZt four dimensions. Different STED technical advances and novel STED probes are discussed with their respective application in biological subcellular imaging. This review may serve as a practical guide for choosing a suitable approach to the advanced STED super-resolution imaging.

中文翻译：

用于四个维度生物成像的受激发射损耗显微镜：综述

受激发射损耗 (STED) 显微镜可在活细胞中实现高横向和轴向分辨率、长期成像。在这里，我们回顾了 STED 显微镜的最新技术进展，重点是XYZt四个维度的分辨率和测量范围。讨论了不同的 STED 技术进步和新型 STED 探针及其在生物亚细胞成像中的各自应用。这篇综述可以作为选择合适的先进 STED 超分辨率成像方法的实用指南。

更新日期：2021-03-13

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