当前位置：
X-MOL 学术
›
J. Surfactants Deterg.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Incorporation of Membrane Proteins Into Bicontinuous Microemulsions Through Winsor-III System-Based Extraction
Journal of Surfactants and Detergents ( IF 1.6 ) Pub Date : 2021-03-14 , DOI: 10.1002/jsde.12500 Douglas G. Hayes 1 , Divina B. Anunciado 2, 3 , Ran Ye 1 , Rachel N. D. Williams 2 , Hugh M. O'Neill 2 , Sai Venkatesh Pingali 2 , Volker S. Urban 2
Journal of Surfactants and Detergents ( IF 1.6 ) Pub Date : 2021-03-14 , DOI: 10.1002/jsde.12500 Douglas G. Hayes 1 , Divina B. Anunciado 2, 3 , Ran Ye 1 , Rachel N. D. Williams 2 , Hugh M. O'Neill 2 , Sai Venkatesh Pingali 2 , Volker S. Urban 2
Affiliation
|
The membrane proteins (MP) α-synuclein (ASYN) and bacteriorhodopsin (BR) were readily incorporated into bicontinuous microemulsions (BμEs) formed by two microemulsion systems: water/heptane/Aerosol-OT (AOT)/CK-2,13 and water/dodecane/sodium dodecyl sulfate (SDS)/1-pentanol. (CK-2,13 is an alkyl ethoxylate possessing two alkyl tail groups of carbon chain length 2 and 13 and an average degree of ethoxylation of 5.6.) MP were encapsulated in BμEs through preparation of Winsor-III systems at optimal salinity, with the anionic surfactants AOT and SDS providing the driving force for extraction. Dissolution of ASYN in BμEs greatly increased the former's α-helicity, similar to ASYN's behavior in the presence of biomembranes, while BμE- and vesicle-encapsulated BR possessed similar secondary structure. Small-angle neutron scattering (SANS) results clearly demonstrated the direct interaction of MP with the surfactants, resulting in a decrease of surface area per volume for surfactant monolayers due to decreased surfactant efficiency. The SANS signal for ASYN was isolated through the use of neutron contrast matching for the surfactants through partial deuteration of water and oil, one of the first reports of contrast matching for BμEs in the literature. The SANS results of the contrast-matched sample reflected similar aggregation for ASYN in BμEs as was reported previously for vesicles and SDS solution. This study demonstrates the potential use of BμEs as MP host systems for conducting biochemical reactions such as the conversion of sunlight into adenosine triphosphate by BR and studying the fundamental behavior of MP, such as the role of ASYN dysfunction in Parkinson's disease, as well as for isolation and purification of MP via Winsor-III-based extraction.
中文翻译：
通过基于 Winsor-III 系统的提取将膜蛋白掺入双连续微乳液中
膜蛋白 (MP) α-突触核蛋白 (ASYN) 和细菌视紫红质 (BR) 很容易掺入由两种微乳液系统形成的双连续微乳液 (BμEs) 中：水/庚烷/气溶胶-OT (AOT)/CK-2,13 和水/十二烷/十二烷基硫酸钠（SDS）/1-戊醇。（CK-2,13 是一种烷基乙氧基化物，具有两个碳链长度为 2 和 13 的烷基尾基，平均乙氧基化度为 5.6。）通过在最佳盐度下制备 Winsor-III 系统，将 MP 封装在 BμEs 中，具有阴离子表面活性剂 AOT 和 SDS 为萃取提供驱动力。ASYN 在 BμE 中的溶解大大增加了前者的 α-螺旋度，类似于 ASYN 在生物膜存在下的行为，而 BμE 和囊泡封装的 BR 具有相似的二级结构。小角中子散射 (SANS) 结果清楚地证明了 MP 与表面活性剂的直接相互作用，由于表面活性剂效率降低，导致表面活性剂单层的单位体积表面积减少。ASYN 的 SANS 信号是通过对水和油的部分氘化使用表面活性剂的中子对比匹配来分离的，这是文献中关于 BμE 对比匹配的最早报道之一。对比匹配样品的 SANS 结果反映了 BμE 中 ASYN 的类似聚集，正如之前报道的囊泡和 SDS 溶液一样。这项研究证明了 BμEs 作为 MP 宿主系统的潜在用途，用于进行生化反应，例如通过 BR 将阳光转化为三磷酸腺苷，并研究 MP 的基本行为，
更新日期：2021-03-14
中文翻译：
通过基于 Winsor-III 系统的提取将膜蛋白掺入双连续微乳液中
膜蛋白 (MP) α-突触核蛋白 (ASYN) 和细菌视紫红质 (BR) 很容易掺入由两种微乳液系统形成的双连续微乳液 (BμEs) 中：水/庚烷/气溶胶-OT (AOT)/CK-2,13 和水/十二烷/十二烷基硫酸钠（SDS）/1-戊醇。（CK-2,13 是一种烷基乙氧基化物，具有两个碳链长度为 2 和 13 的烷基尾基，平均乙氧基化度为 5.6。）通过在最佳盐度下制备 Winsor-III 系统，将 MP 封装在 BμEs 中，具有阴离子表面活性剂 AOT 和 SDS 为萃取提供驱动力。ASYN 在 BμE 中的溶解大大增加了前者的 α-螺旋度，类似于 ASYN 在生物膜存在下的行为，而 BμE 和囊泡封装的 BR 具有相似的二级结构。小角中子散射 (SANS) 结果清楚地证明了 MP 与表面活性剂的直接相互作用，由于表面活性剂效率降低，导致表面活性剂单层的单位体积表面积减少。ASYN 的 SANS 信号是通过对水和油的部分氘化使用表面活性剂的中子对比匹配来分离的，这是文献中关于 BμE 对比匹配的最早报道之一。对比匹配样品的 SANS 结果反映了 BμE 中 ASYN 的类似聚集，正如之前报道的囊泡和 SDS 溶液一样。这项研究证明了 BμEs 作为 MP 宿主系统的潜在用途，用于进行生化反应，例如通过 BR 将阳光转化为三磷酸腺苷，并研究 MP 的基本行为，
京公网安备 11010802027423号