Chronic wounds result from the failure of the normal wound healing process. Any delay during the tissue repair process could be defined as chronic wound healing, potentially having a highly detrimental impact on human health. To face this problem, in the last years, the use of different technologies alternative to therapeutic agents is gaining more attention. The Helix aspersa snail slime‐based products are increasingly being used for skin injury, thanks to their ability to make tissue repair processes faster. To date, a comprehensive overview of pure snail slime metabolome is not available. Besides, Au nanoparticles (AuNPs) technology is spreading rapidly in the medical environment, and the search for AuNPs “green” synthetic routes that involve natural products as precursor agents is demanded, alongside with a deep comprehension of the kind of species that actively take part in synthesis and product stabilization. The aim of this work is to characterize the metabolic profile of a pure snail slime sample, by an untargeted high‐resolution mass spectrometry‐based analysis. In addition, insights on AuNPs synthesis and stabilization by the main components of pure snail slime used to induce the synthesis were obtained. The untargeted analysis provided a large list of important classes of metabolites, that is, fatty acid derivatives, amino acids and peptides, carbohydrates and polyphenolic compounds that could be appreciated in both samples of slime, with and without AuNPs. Moreover, a direct comparison of the obtained results suggests that mostly nitrogen and sulfur‐bearing metabolites take part in the synthesis and stabilization of AuNPs.

中文翻译：

---

使用 MALDI FT-ICR MS 对纯蜗牛泥和蜗牛泥诱导的 Au 纳米粒子代谢组进行非靶向分析

慢性伤口是由于正常伤口愈合过程失败造成的。组织修复过程中的任何延迟都可以定义为慢性伤口愈合，可能对人类健康产生非常不利的影响。面对这个问题，在过去几年中，使用不同的技术替代治疗剂越来越受到关注。该花园蜗牛基于蜗牛粘液的产品越来越多地用于治疗皮肤损伤，因为它们能够加快组织修复过程。迄今为止，还没有关于纯蜗牛粘液代谢组的全面概述。此外，金纳米粒子（AuNPs）技术在医疗环境中迅速普及，需要探索以天然产物为前驱体的金纳米粒子“绿色”合成路线，同时深入了解积极参与的物种种类。在合成和产品稳定方面。这项工作的目的是通过基于非靶向高分辨率质谱的分析来表征纯蜗牛粘液样品的代谢特征。此外，还获得了用于诱导合成的纯蜗牛粘液的主要成分对 AuNPs 合成和稳定的见解。非靶向分析提供了大量重要代谢物类别，即脂肪酸衍生物、氨基酸和肽、碳水化合物和多酚化合物，可以在含有和不含 AuNP 的两种粘液样品中进行分析。此外，对所得结果的直接比较表明，大多数含氮和硫的代谢物参与了 AuNPs 的合成和稳定化。