In this work, a green strategy was developed to prepare molecularly imprinted polymers functionalized magnetic carbon nanotubes in aqueous phase under mild conditions for cyclic adenosine monophosphate. Thanks to water solubility of chitosan, a natural polysaccharide which is rich in amino and hydroxyl groups, provided the feasibility to synthesize the green molecularly imprinted polymers for water soluble template in aqueous media. Coupled with high-performance liquid chromatography, the method exhibited a short equilibrium time (6 min), high adsorption capacity (22.42 μg/mg), high magnetic susceptibility, and good selectivity to template molecule with the imprinting factor of 2.94. A good linearity in the range of 0.020-3.0 mg/mL for target was obtained with a correlation coefficient of 0.9998. The limit of detection (signal-to-noise ratio = 3) and limit of quantitation (signal-to-noise ratio = 10) of the magnetic solid phase extraction method for cyclic adenosine monophosphate were 5 and 15 ng/mg, respectively. And the practical application of chitosan-based molecularly imprinted polymers as adsorbent to isolate and determine cyclic adenosine monophosphate in real natural samples (winter jujube) was demonstrated.

中文翻译：

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亲水性分子印迹聚合物功能化磁性碳纳米管用于从冬枣中选择性提取环磷酸腺苷

在这项工作中，开发了一种绿色策略，在温和条件下制备分子印迹聚合物功能化的磁性碳纳米管在水相中制备环磷酸腺苷。由于壳聚糖（一种富含氨基和羟基的天然多糖）的水溶性，为在水性介质中合成用于水溶性模板的绿色分子印迹聚合物提供了可行性。结合高效液相色谱，该方法表现出平衡时间短（6 分钟）、高吸附容量（22.42 μg/mg）、高磁化率和良好的模板分子选择性，印迹因子为2.94。目标在 0.020-3.0 mg/mL 范围内具有良好的线性，相关系数为 0.9998。环磷酸腺苷磁性固相萃取方法的检测限（信噪比= 3）和定量限（信噪比= 10）分别为5和15 ng/mg。并展示了基于壳聚糖的分子印迹聚合物作为吸附剂在实际天然样品（冬枣）中分离和测定环磷酸腺苷的实际应用。