Inositol polyphosphates (IPs) and inositol pyrophosphates (PP–IPs) regulate diverse cellular processes in eukaryotic cells. IPs and PP–IPs are highly negatively charged and exert their biological effects by interacting with specific protein targets. Studies performed predominantly in mammalian cells and model yeasts have shown that IPs and PP–IPs modulate target function through allosteric regulation, by promoting intra‐ and intermolecular stabilization and, in the case of PP–IPs, by donating a phosphate from their pyrophosphate (PP) group to the target protein. Technological advances in genetics have extended studies of IP function to microbial pathogens and demonstrated that disrupting PP–IP biosynthesis and PP–IP‐protein interaction has a profound impact on pathogenicity. This review summarises the complexity of IP‐mediated regulation in eukaryotes, including microbial pathogens. It also highlights examples of poor conservation of IP–protein interaction outcome despite the presence of conserved IP‐binding domains in eukaryotic proteomes.

中文翻译：

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多磷酸肌醇-蛋白质相互作用：对微生物致病性的影响

多磷酸肌醇 (IPs) 和焦磷酸肌醇 (PP-IPs) 调节真核细胞中的多种细胞过程。IPs 和 PP-IPs 带高负电荷，并通过与特定蛋白质靶标相互作用发挥其生物学效应。主要在哺乳动物细胞和模型酵母中进行的研究表明，IPs 和 PP-IPs 通过变构调节调节靶功能，通过促进分子内和分子间的稳定，在 PP-IPs 的情况下，通过从它们的焦磷酸盐中提供磷酸盐（PP ) 组到目标蛋白质。遗传学的技术进步已将 IP 功能的研究扩展到微生物病原体，并证明破坏 PP-IP 生物合成和 PP-IP-蛋白质相互作用对致病性具有深远的影响。本综述总结了 IP 介导的真核生物调控的复杂性，包括微生物病原体。它还强调了尽管真核蛋白质组中存在保守的 IP 结合结构域，但 IP-蛋白质相互作用结果保守性较差的例子。