Here we report a new robust nicotinamide dinucleotide phosphate cofactor analog (carba-NADP⁺) and its acceptance by many enzymes in the class of oxidoreductases. Replacing one ribose oxygen with a methylene group of the natural NADP⁺ was found to enhance stability dramatically. Decomposition experiments at moderate and high temperatures with the cofactors showed a drastic increase in half-life time at elevated temperatures since it significantly disfavors hydrolysis of the pyridinium-N−glycoside bond. Overall, more than 27 different oxidoreductases were successfully tested, and a thorough analytical characterization and comparison is given. The cofactor carba-NADP⁺ opens up the field of redox-biocatalysis under harsh conditions.

中文翻译：

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carba 烟酰胺腺嘌呤二核苷酸磷酸盐：氧化还原生物催化的强大辅因子

在这里，我们报告了一种新的强大的烟酰胺二核苷酸磷酸辅因子类似物（carba-NADP ⁺）及其被氧化还原酶类中的许多酶所接受。研究发现，用天然 NADP ⁺的亚甲基取代一个核糖氧可显着增强稳定性。使用辅因子在中温和高温下进行的分解实验表明，高温下的半衰期急剧增加，因为它显着不利于吡啶鎓-N-糖苷键的水解。总体而言，成功测试了超过 27 种不同的氧化还原酶，并给出了全面的分析表征和比较。辅因子 carba-NADP ⁺开辟了恶劣条件下氧化还原生物催化领域。