This study was carried out to evaluate the population growth and egg ratio of Brachionus rotundiformis by feeding with three different diets, i.e., live microalgae Nannochloropsis sp., Baker’s yeast, and powdered spirulina. A total of nine conical flasks of 1 L volume were divided into three treatment groups for determining the feeding performance for eight consecutive days. Each experimental group was stocked with B. rotundiformis at an initial density of 10 ind. mL⁻¹. Water temperature, pH, salinity, and light were maintained at (24–29) °C, (7.5–8.5), 30 ppt, and 4000 lx, respectively. Feed was provided twice per day at 9:00 AM and 7:00 PM. The highest mean (±SE) population growth of rotifer was achieved at the end of the culture period (on the 8th day) fed live Nannochloropsis sp. (122 ± 12 ind. mL⁻¹), which was significantly higher compared to the Baker’s yeast and powdered spirulina (p < 0.05). The mean egg ratio of B. rotundiformis fed three diets was significantly differ at different rearing periods (p > 0.05). The highest mean (±SE) egg ratio of B. rotundiformis was observed in the live Nannochloropsis fed group (0.89 ± 0.01) at the end of the rearing period, which was significantly higher than that of Baker’s Yeast and powdered Spirulina fed group (p < 0.05). Among the three diets, live Nannochloropsis had shown the dietary superiority over Baker’s yeast and powdered spirulina in culturing B. rotundiformis. The results of this study suggest that live Nannochloropsis could be the most suitable diet for the laboratory culture of B. rotundiformis.

这项研究是通过饲喂三种不同的饮食，即活的微藻Nannochloropsis sp。，Baker's酵母和粉状螺旋藻，来评估轮枝Brachionus rotundiformis的种群生长和卵比。将总共​​9个1 L容量的锥形烧瓶分成三个处理组，以确定连续八天的进食性能。每个实验组均以初始密度为10 ind的轮状芽孢杆菌进行放养。毫升^-1。水温，pH，盐度和光照分别维持在（24–29）°C，（7.5–8.5），30 ppt和4000 lx。每天在9:00 AM和7:00 PM提供两次提要。在活的Nannochloropsis sp。的培养期结束时（第8天），轮虫达到了最高的平均（±SE）种群增长。（122±12 ind。mL ^-1），比贝克酵母和粉状螺旋藻高得多（p  <0.05）。三种日粮饲喂的轮状芽孢杆菌的平均卵比在不同的饲养时期有显着差异（p  > 0.05）。在现场观察到轮状芽孢杆菌的最高平均卵子率（±SE）在饲养期结束时，Nannochloropsis喂养组（0.89±0.01），显着高于Baker's Yeast和螺旋藻粉喂养组（p  <0.05）。在这三种饮食中，活的拟南芥在培养轮状芽孢杆菌中显示出优于贝克酵母和螺旋藻粉的饮食优势。这项研究的结果表明，活的拟南芥可能是轮状芽孢杆菌实验室培养的最合适饮食。