The natural pigment fucoxanthin has attracted global attention because of its superior antioxidant properties. The haptophyte marine microalgae Pavlova spp. are assumed to be promising industrial fucoxanthin producers as their lack of a cell wall could facilitate the commercialization of cultured cells as a whole food. This study screened promising Pavlova strains with high fucoxanthin content to develop an outdoor cultivation method for fucoxanthin production. Initial laboratory investigations of P. pinguis NBRC 102807, P. lutheri NBRC 102808, and Pavlova sp. OPMS 30543 identified OPMS 30543 as having the highest fucoxanthin content. The culture conditions were optimized for OPMS 30543. Compared with f/2 and Walne’s media, the use of Daigo’s IMK medium led to the highest biomass production and highest fucoxanthin accumulation. The presence of seawater elements in Daigo’s IMK medium was necessary for the growth of OPMS 30543. OPMS 30543 was then cultured outdoors using acrylic pipe photobioreactors, a plastic bag, an open tank, and a raceway pond. Acrylic pipe photobioreactors with small diameters enabled the highest biomass production. Using an acrylic pipe photobioreactor with 60-mm diameter, a fucoxanthin productivity of 4.88 mg/L/day was achieved in outdoor cultivation. Thus, this study demonstrated the usefulness of Pavlova sp. OPMS 30543 for fucoxanthin production in outdoor cultivation.

天然色素岩藻黄质因其卓越的抗氧化性能而受到全球关注。触藻海洋微藻Pavlova spp。被认为是有前途的工业岩藻黄质生产商，因为它们缺乏细胞壁可以促进培养细胞作为整体食品的商业化。该研究筛选了具有高岩藻黄质含量的有前途的巴甫洛娃菌株，以开发一种用于生产岩藻黄质的户外栽培方法。P. pinguis NBRC 102807、P. lutheri NBRC 102808 和Pavlova 的初步实验室调查sp. OPMS 30543 确定 OPMS 30543 具有最高的岩藻黄质含量。针对 OPMS 30543 优化了培养条件。与 f/2 和 Walne 培养基相比，使用 Daigo 的 IMK 培养基导致最高的生物量产量和最高的岩藻黄质积累。Daigo 的 IMK 培养基中海水元素的存在是 OPMS 30543 生长所必需的。然后使用丙烯酸管光生物反应器、塑料袋、开放式水箱和跑道池在户外培养 OPMS 30543。小直径的丙烯酸管光生物反应器可实现最高的生物质产量。使用直径为 60 毫米的丙烯酸管光生物反应器，在室外栽培中实现了 4.88 毫克/升/天的岩藻黄质生产率。因此，这项研究证明了巴甫洛娃的有用性sp. OPMS 30543 用于户外栽培中岩藻黄质的生产。