Haematococcus pluvialis is known as the richest natural source of carotenoid astaxanthin, whose synthesis is related to changes in cells according its complex life cycle and the availability of nutrients present in the culture media. This study’s objective was to evaluate the influence of different culture media under photoautotrophic and mixotrophic conditions on the growth, life cycle, biochemical composition, and astaxanthin production in H. pluvialis. Cultures were performed using the following media: KM1, MM2, Provasoli, modified Provasoli, and how to control the BBM, all kept at 24 ± 1 °C, with constant aeration, light/dark photoperiod 24:0 and irradiance of 60 μmol photons/m²/s. Growth and life cycle were evaluated by daily cell counts under an optical microscope; values of maximum cell density (MCD) and dry biomass were also assessed. Biochemical analyses were chlorophyll (a and b), astaxanthin, total proteins, and fatty acids. Cultivation under photoautotrophic conditions in BBM medium showed higher concentration of chlorophylls a (33 ± 4.0 μg/mL) and b (18 ± 1.6 μg/mL) and protein (62.7 ± 3.38%). Cultivation in modified Provasoli medium, under mixotrophic conditions, showed higher MCD (84.5 ± 11.7), biomass production (4.9 ± 0.0004 mg/mL), fatty acid methyl esters, and astaxanthin productivity (9.28 ± 0.4 mg/L/day). These results demonstrate that the culture media and the culture conditions to which H. pluvialis is submitted stimulate specific metabolic and biosynthetic pathways of the cells, which directly affect the biochemical composition of the microalgae. Modified Provasoli medium proved to be the most efficient in increasing astaxanthin productivity for this species, as it has shown better growth parameters and biomass production.

雨生红球菌是类胡萝卜素虾青素的最丰富的天然来源，其合成与细胞的变化有关，这取决于其复杂的生命周期和培养基中营养素的可用性。这项研究的目的是评估光养养和混合养养条件下不同培养基对雨生红球藻生长，生命周期，生化成分和虾青素生产的影响。使用以下培养基进行培养：KM1，MM2，Provasoli，改良的Provasoli以及如何控制BBM，均保持在24±1°C，恒定曝气，明/暗光周期24：0和60μmol光子辐照度/米²/ s。在光学显微镜下通过每日细胞计数评估生长和生命周期；还评估了最大细胞密度（MCD）和干生物量的值。生化分析是叶绿素（a和b），虾青素，总蛋白和脂肪酸。在BBM培养基中光养养条件下的培养显示出较高浓度的叶绿素a（33±4.0μg/ mL）和b（18±1.6μg/ mL）和蛋白质（62.7±3.38％）。在混合营养条件下，在改良的Provasoli培养基中培养显示出较高的MCD（84.5±11.7），生物量产生（4.9±0.0004 mg / mL），脂肪酸甲酯和虾青素生产率（9.28±0.4 mg / L / day）。这些结果表明，培养基和培养条件对幽门螺杆菌被提交刺激细胞的特定代谢和生物合成途径，这直接影响微藻的生物化学组成。事实证明，改良的Provasoli培养基对于提高该物种的虾青素产量是最有效的，因为它显示了更好的生长参数和生物量生产。