The tire track eel, Mastacembelus favus, is an economically important freshwater fish in mainland Southeast Asia. Understanding the spatial population structure is important to design an effective and efficient management of the species. The aim of this study was to investigate the genetic diversity and structure of M. favus populations. In total, 519 individuals from 30 locations across its distribution range were genotyped using a set of eight polymorphic microsatellite loci. Results showed that populations in Mekong and Chao Phraya exhibited higher genetic variability than those from Gulf of Thailand and Malay–Thai Peninsula. Clustering results revealed two evolutionarily isolated groups geographically separated at Northeastern Cambodia; the more widespread group was further divided into two subclusters. The spatial genetic variation and structure of its populations are attributed to the evolutionary history and population dynamics of the species. The study provides important information for the management and conservation of M. favus in Southeast Asia.

轮胎track鳗，Mastacembelus favus，是东南亚大陆上一种经济上重要的淡水鱼。了解空间种群结构对于设计该物种的有效管理非常重要。这项研究的目的是调查青霉的遗传多样性和结构。人口。使用一组八个多态微卫星基因座，对来自其分布范围内30个地点的519个人进行了基因分型。结果表明，湄公河和湄南河的种群遗传变异性高于泰国湾和马来泰国半岛的种群。聚类结果表明，柬埔寨东北部有两个地理上分离的进化上孤立的群体。范围更广的组又被分为两个子组。其种群的空间遗传变异和结构归因于该物种的进化历史和种群动态。该研究为东南亚地区青霉的管理和保护提供了重要的信息。