JBIC Journal of Biological Inorganic Chemistry ( IF 2.7 ) Pub Date : 2021-03-15 , DOI: 10.1007/s00775-021-01860-0 Ayşegül Tekbaba 1 , Sena Çağatay Özpınar 1 , Hatice Tunca 1 , Tuğba Ongun Sevindik 1 , Ali Doğru 1 , Armağan Günsel 2 , Ahmet T Bilgiçli 2 , M Nilüfer Yarasir 2
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Abstract
In this study, the chemical and algicidal properties of the newly synthesized compound (2) were evaluated and its algal oxidative effects were determined in Arthrospira platensis and Chlorella vulgaris. First, we have reported on the synthesis and characterization of highly water-soluble copper (II) phthalocyanine (2), containing sodium 2-mercaptoethanesulfonate (2) substituents at the peripheral positions. Some spectroscopic techniques were used to characterize the new synthesized compound (2). In terms of biological properties, C. vulgaris were more tolerance to compound (2) than A. platensis depending to growth parameters. When SOD (Superoxide dismutase) activity significantly increased at 0.25 ppb and 1.5 ppb concentrations in A. platensis cultures, it increased at 6 ppb concentration in C. vulgaris cultures. GR (Glutathione reductase) activity decreased at 1 ppb and 1.5 ppb concentrations while APX (Ascorbate peroxidase) activity did not show a significant change at any concentrations in A. platensis cultures. GR activity showed a significant increase at 6 ppb concentration, while APX activity increased at all concentrations compared to control in C. vulgaris cultures. MDA (malondialdehyde) and H2O2 content decreased at 1 and 1.5 ppb concentrations but there were significant increases in the proline content at all concentrations compared to the control in A. platensis. MDA, H2O2 and free proline contents showed a significant increase at 0.5 ppb concentration in C. vulgaris. In conclusion, compound (2) have algicidal effects, and also it causes to oxidative stress in these organisms.
Graphic abstract
中文翻译：
含磺酸基水溶性铜酞菁的合成、表征及藻类氧化作用研究
抽象的
在本研究中，评估了新合成的化合物（2）的化学和杀藻特性，并测定了其在钝顶节旋藻和普通小球藻中的藻类氧化作用。首先，我们报道了在外围位置含有 2-巯基乙磺酸钠(2)取代基的高水溶性铜 (II) 酞菁(2)的合成和表征。使用一些光谱技术来表征新合成的化合物(2) 。就生物学特性而言，根据生长参数，普通念珠菌比钝顶曲霉对化合物(2)具有更高的耐受性。当钝顶孢菌培养物中 SOD（超氧化物歧化酶）活性在 0.25 ppb 和 1.5 ppb 浓度下显着增加时，在普通弯曲菌培养物中，当浓度为 6 ppb 时，SOD（超氧化物歧化酶）活性显着增加。在钝顶菌培养物中，GR（谷胱甘肽还原酶）活性在 1 ppb 和 1.5 ppb 浓度下降低，而 APX（抗坏血酸过氧化物酶）活性在任何浓度下均未显示出显着变化。在普通念珠菌培养物中，与对照相比，GR 活性在 6 ppb 浓度下显着增加，而 APX 活性在所有浓度下均增加。与钝顶菌中的对照相比，MDA（丙二醛）和H 2 O 2含量在1 和1.5 ppb 浓度下下降，但在所有浓度下脯氨酸含量均显着增加。在普通念珠菌中，MDA、H 2 O 2和游离脯氨酸含量在0.5 ppb浓度下显着增加。 总之，化合物(2)具有杀藻作用，并且还引起这些生物体的氧化应激。
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